CHAPTER 13: POCT for Nucleic Acids by Using Colorimetric Nanoprobes

Shiyao Wang, Thomas M.H. Lee

Research output: Chapter in book / Conference proceedingChapter in an edited book (as author)Academic researchpeer-review

Abstract

Nucleic acid detection in point-of-care settings would bring tremendous benefits to early diagnosis. Colorimetric nanoprobes have proved to be promising for point-of-care testing (POCT) thanks to their simple result readout (qualitative visual and/or quantitative transmittance/absorbance readout). In this chapter, two types of colorimetric nanoprobes (i.e., gold nanoparticles and enzyme mimetics) for nucleic acid testing are introduced. To realize highly sensitive detection, some of these colorimetric assays have been coupled with nucleic acid amplification, which can be classified into thermocycling and isothermal amplification reactions. Current challenges for widespread applications of point-of-care nucleic acid testing are discussed.

Original languageEnglish
Title of host publicationDetection Methods in Precision Medicine
EditorsMengsu Yang, Michael Thompson
PublisherRoyal Society of Chemistry
Pages279-302
Number of pages24
Edition18
DOIs
Publication statusPublished - 14 Dec 2020

Publication series

NameRSC Detection Science
Number18
Volume2021-January
ISSN (Print)2052-3068
ISSN (Electronic)2052-3076

ASJC Scopus subject areas

  • Bioengineering
  • Pathology and Forensic Medicine
  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pollution
  • Biochemistry, medical

Cite this