Ceruloplasmin promotes iron uptake rather than release in BT325 cells

Zhong Ming Qian, Yin King Tsoi, Y. Ke, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

This study was initiated to investigate the mechanism of ceruloplasmin (CP)-mediated iron release from brain cells using BT325 cells (a glioblastoma cell line); however, negative results were obtained. The BT325 cells were preloaded with 1 μM59Fe2+in sucrose (pH 5.8) for 60 min, and then with CP (0-300 μg/ml) for 30 min at 37°C. The addition of CP, either at low (25 μg/ml) or high (300 μg/ml) concentrations, did not lead to a significant change in iron release from ironloaded BT325 cells. No significant difference in total iron of cells was found between all CP treated groups and the control (P>0.05). Although apotransferrin (apoTf) was shown to have a role in iron release from the cells, the effect of apoTf was not significantly affected by the addition of different concentrations of CP. When the cells were incubated with 1 μM59Fe2+in the presence of varying amounts of CP for 30 min at 37°C, it was found that CP increased iron uptake. The total iron uptake by BT325 cells in CP treatment groups (25, 75, 150, 300 μg/ml) was significantly higher than that in the control (no CP addition) (all P<0.01). Furthermore, in contrast to our expectation, CP was shown to promote significantly iron uptake in not only iron-sufficient but also iron-deficient cells. These results showed that CP had a role in iron uptake rather than release in BT325 cells.
Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalExperimental Brain Research
Volume140
Issue number3
DOIs
Publication statusPublished - 22 Sep 2001

Keywords

  • Brain iron transport
  • BT325 cells
  • Ceruloplasmin
  • Iron uptake
  • Iron-sufficient and iron-deficient cells

ASJC Scopus subject areas

  • Neuroscience(all)

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