Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study

Jerry S. Cheung; April M. Chow; Edward S. Hui; Jian Yang; Hung Fat Tse; Ed X. Wu

doi:10.1109/IEMBS.2006.259696

Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study

Jerry S. Cheung, April M. Chow, Edward S. Hui, Jian Yang, Hung Fat Tse, Ed X. Wu

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

5 Citations (Scopus)

Abstract

MRI plays an expanding role in stem cell therapies. The non-invasive nature and high spatial resolution of MR imaging make MR imaging a powerful tool to investigate biologic processes at the molecular and cellular level in vivo longitudinally. Quantitative detection of stem cells after transplantation may allow assessment of stem cell localization and migration, and monitoring of the therapeutic effectiveness of stem cell therapy. In this study, we present a technique for MR quantification of magnetically labeled mouse embryonic stem cells distributed or injected in agarose gel phantoms. Apparent transverse relaxation rate enhancements (ΔR2*) were measured by gradient echo sequences. The linear relationship between ΔR2* and the concentration of USPIO-labeled mouse embryonic stem cells was observed and used for quantifying cell density and cell number after injection or transplantation. The MRI acquisition and analysis protocol were validated by good agreement between actual cell numbers and MRI-estimated cell numbers over a wide range of cell numbers. This MR technique for cell number and cell density quantification is applicable to future in vivo studies.

Original language	English
Title of host publication	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	476-479
Number of pages	4
ISBN (Print)	1424400325, 9781424400324
DOIs	https://doi.org/10.1109/IEMBS.2006.259696
Publication status	Published - Dec 2006
Externally published	Yes
Event	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States Duration: 30 Aug 2006 → 3 Sept 2006

Publication series

Name	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)	0589-1019

Conference

Conference	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Country/Territory	United States
City	New York, NY
Period	30/08/06 → 3/09/06

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

More information

10.1109/IEMBS.2006.259696

Cite this

Cheung, J. S., Chow, A. M., Hui, E. S., Yang, J., Tse, H. F., & Wu, E. X. (2006). Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study. In 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 (pp. 476-479). Article 4029708 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IEMBS.2006.259696

Cheung, Jerry S. ; Chow, April M. ; Hui, Edward S. et al. / Cell number quantification of USPIO-labeled stem cells by MRI : An in vitro study. 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06. Institute of Electrical and Electronics Engineers Inc., 2006. pp. 476-479 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).

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abstract = "MRI plays an expanding role in stem cell therapies. The non-invasive nature and high spatial resolution of MR imaging make MR imaging a powerful tool to investigate biologic processes at the molecular and cellular level in vivo longitudinally. Quantitative detection of stem cells after transplantation may allow assessment of stem cell localization and migration, and monitoring of the therapeutic effectiveness of stem cell therapy. In this study, we present a technique for MR quantification of magnetically labeled mouse embryonic stem cells distributed or injected in agarose gel phantoms. Apparent transverse relaxation rate enhancements (ΔR2*) were measured by gradient echo sequences. The linear relationship between ΔR2* and the concentration of USPIO-labeled mouse embryonic stem cells was observed and used for quantifying cell density and cell number after injection or transplantation. The MRI acquisition and analysis protocol were validated by good agreement between actual cell numbers and MRI-estimated cell numbers over a wide range of cell numbers. This MR technique for cell number and cell density quantification is applicable to future in vivo studies.",

author = "Cheung, {Jerry S.} and Chow, {April M.} and Hui, {Edward S.} and Jian Yang and Tse, {Hung Fat} and Wu, {Ed X.}",

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year = "2006",

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doi = "10.1109/IEMBS.2006.259696",

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Cheung, JS, Chow, AM, Hui, ES, Yang, J, Tse, HF & Wu, EX 2006, Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study. in 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06., 4029708, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 476-479, 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06, New York, NY, United States, 30/08/06. https://doi.org/10.1109/IEMBS.2006.259696

Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study. / Cheung, Jerry S.; Chow, April M.; Hui, Edward S. et al.
28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06. Institute of Electrical and Electronics Engineers Inc., 2006. p. 476-479 4029708 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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AU - Tse, Hung Fat

AU - Wu, Ed X.

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ER -

Cheung JS, Chow AM, Hui ES, Yang J, Tse HF, Wu EX. Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study. In 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06. Institute of Electrical and Electronics Engineers Inc. 2006. p. 476-479. 4029708. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings). doi: 10.1109/IEMBS.2006.259696

Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study

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