Cell cycle phase-dependent effect of retinoic acid on the induction of granulocytic differentiation in HL-60 promyelocytic leukemia cells. Evidence for sphinganine potentiation of retinoic acid-induced differentiation

Eric Ka Wai Hui, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

28 Citations (Scopus)


The efficiency of retinoic acid (RA)-induced differentiation was dependent on the position of HL-60 cells in the cell cycle. Our results demonstrated that cells at the G1/S border were more efficiently induced to differentiate by short exposure to RA than cells at other phases of the cell cycle. Synchronization of cells in G1/S phase by aphidicolin (APH) or mimosine (MIMO) increased the sensitivity of cells to RA short exposure treatment. Pretreatment with sphinganine (SP), a protein kinase C (PKC) inhibitor, potentiated RA-induced cell differentiation. By cell cycle analysis, SP was found to block the cell progression through the G1/S phase. Consequently, cells accumulated in the G1/S phase of the cell cycle. The present data therefore suggest a possible mechanism of action of SP to enhance RA-induced differentiation.
Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalFEBS Letters
Issue number2
Publication statusPublished - 1 Mar 1993
Externally publishedYes


  • Cell cycle
  • Granulocytic differentiation
  • Retinoic acid
  • Sphinganine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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