Cardiac parameters analysis for zebrafish heart regeneration based on highfrequency ultrasound imaging

Yanyan Yu, Ching Ling Lien, K. Kirk Shung, Lei Sun

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

1 Citation (Scopus)


Zebrafish can fully regenerate their myocardium after up to 20% ventricularresection within 2 months without evidence of scar tissues. The extraordinaryregenerative abilities provide a significant model system to study theactivation of the regenerative potential of human heart tissue. In order tocharacterize cardiac functions of zebrafish during heart regeneration, we usedhigh-frame-rate high-frequency ultrasound system with the capabilities of 75 MHzB-mode imaging to monitor real-time cardiac parameters. Longitudinal in vivoexperiments was carried out to capture echocardiographic images from individualfishes. A pilot study on 6 fish over 30 days post amputation (dpa) was performedusing this technique. A total of over 400 videos were captured. To process thelarge video data, an automatic image segmentation algorithm was developed. Byusing information obtained from temporal decorrelation of the B-mode imagesequence, the epicardium is determined in a region-based level set frameworkcombined with shape priors and Kalman filtering. Afterwards, the size of theheart was estimated frame by frame using the outlined epicardium to obtain itsdynamic variation. Subsequently, the maximum and minimum of the heart size wasused to calculate ejection fraction (EF). The time course of the mean EF (n=6)with a V shape indicates the strong ability of zebrafish to recovery cardiacfunctions along its morphological regeneration.
Original languageEnglish
Title of host publication2010 IEEE International Ultrasonics Symposium, IUS 2010
Number of pages4
Publication statusPublished - 1 Dec 2010
Event2010 IEEE International Ultrasonics Symposium, IUS 2010 - San Diego, CA, United States
Duration: 11 Oct 201014 Oct 2010


Conference2010 IEEE International Ultrasonics Symposium, IUS 2010
Country/TerritoryUnited States
CitySan Diego, CA


  • automatic segmentation
  • ejection fraction
  • high frequency ultrasound
  • zebrafish heart regeneration

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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