Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation

Luchao Qi; Qi Zhang; Kwok Ho Lam; Ruibiao Guo; Runkang Chen; Jiangbang Huang; Ruoyan Meng; Zhaoyang Wang; Hairong Zheng; Ming Qian

doi:10.1109/ULTSYM.2017.8091686

Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation

Luchao Qi, Qi Zhang, Kwok Ho Lam, Ruibiao Guo, Runkang Chen, Jiangbang Huang, Ruoyan Meng, Zhaoyang Wang, Hairong Zheng, Ming Qian

The Hong Kong Polytechnic University

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

Invasiveness research is an essential step in breast cancer metastasis. The application of high-frequency ultrasound microbeam stimulation (HFUMS) offers a manner of determining the invasion potential of human breast cancer cells by eliciting the elevation of transient cytoplasmic calcium ions (Ca2+). The fluorescent change in invasive breast cancer cells (MDA-MB-231), reflecting Ca2+elevation elicited by HFUMS, is greater compared to that in weakly invasive breast cancer cells (MCF-7). GsMTx4, a selective blocker of mechanosensitive Piezo channels, was used for better understanding of mechanotransduction in HFUMS on cells.

Original language	English
Title of host publication	2017 IEEE International Ultrasonics Symposium, IUS 2017
Publisher	IEEE Computer Society
ISBN (Electronic)	9781538633830
DOIs	https://doi.org/10.1109/ULTSYM.2017.8091686
Publication status	Published - 31 Oct 2017
Event	2017 IEEE International Ultrasonics Symposium, IUS 2017 - Washington, United States Duration: 6 Sept 2017 → 9 Sept 2017

Conference

Conference	2017 IEEE International Ultrasonics Symposium, IUS 2017
Country/Territory	United States
City	Washington
Period	6/09/17 → 9/09/17

Keywords

Calcium fluorescence imaging
High-frequency ultrasound microbeam stimulation
Human breast cells
Piezo

ASJC Scopus subject areas

Acoustics and Ultrasonics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1109/ULTSYM.2017.8091686

Cite this

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title = "Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation",

abstract = "Invasiveness research is an essential step in breast cancer metastasis. The application of high-frequency ultrasound microbeam stimulation (HFUMS) offers a manner of determining the invasion potential of human breast cancer cells by eliciting the elevation of transient cytoplasmic calcium ions (Ca2+). The fluorescent change in invasive breast cancer cells (MDA-MB-231), reflecting Ca2+elevation elicited by HFUMS, is greater compared to that in weakly invasive breast cancer cells (MCF-7). GsMTx4, a selective blocker of mechanosensitive Piezo channels, was used for better understanding of mechanotransduction in HFUMS on cells.",

keywords = "Calcium fluorescence imaging, High-frequency ultrasound microbeam stimulation, Human breast cells, Piezo",

author = "Luchao Qi and Qi Zhang and Lam, {Kwok Ho} and Ruibiao Guo and Runkang Chen and Jiangbang Huang and Ruoyan Meng and Zhaoyang Wang and Hairong Zheng and Ming Qian",

year = "2017",

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doi = "10.1109/ULTSYM.2017.8091686",

language = "English",

booktitle = "2017 IEEE International Ultrasonics Symposium, IUS 2017",

publisher = "IEEE Computer Society",

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note = "2017 IEEE International Ultrasonics Symposium, IUS 2017 ; Conference date: 06-09-2017 Through 09-09-2017",

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Qi, L, Zhang, Q, Lam, KH, Guo, R, Chen, R, Huang, J, Meng, R, Wang, Z, Zheng, H & Qian, M 2017, Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation. in 2017 IEEE International Ultrasonics Symposium, IUS 2017., 8091686, IEEE Computer Society, 2017 IEEE International Ultrasonics Symposium, IUS 2017, Washington, United States, 6/09/17. https://doi.org/10.1109/ULTSYM.2017.8091686

Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation. / Qi, Luchao; Zhang, Qi; Lam, Kwok Ho et al.
2017 IEEE International Ultrasonics Symposium, IUS 2017. IEEE Computer Society, 2017. 8091686.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation

AU - Qi, Luchao

AU - Zhang, Qi

AU - Lam, Kwok Ho

AU - Guo, Ruibiao

AU - Chen, Runkang

AU - Huang, Jiangbang

AU - Meng, Ruoyan

AU - Wang, Zhaoyang

AU - Zheng, Hairong

AU - Qian, Ming

PY - 2017/10/31

Y1 - 2017/10/31

N2 - Invasiveness research is an essential step in breast cancer metastasis. The application of high-frequency ultrasound microbeam stimulation (HFUMS) offers a manner of determining the invasion potential of human breast cancer cells by eliciting the elevation of transient cytoplasmic calcium ions (Ca2+). The fluorescent change in invasive breast cancer cells (MDA-MB-231), reflecting Ca2+elevation elicited by HFUMS, is greater compared to that in weakly invasive breast cancer cells (MCF-7). GsMTx4, a selective blocker of mechanosensitive Piezo channels, was used for better understanding of mechanotransduction in HFUMS on cells.

AB - Invasiveness research is an essential step in breast cancer metastasis. The application of high-frequency ultrasound microbeam stimulation (HFUMS) offers a manner of determining the invasion potential of human breast cancer cells by eliciting the elevation of transient cytoplasmic calcium ions (Ca2+). The fluorescent change in invasive breast cancer cells (MDA-MB-231), reflecting Ca2+elevation elicited by HFUMS, is greater compared to that in weakly invasive breast cancer cells (MCF-7). GsMTx4, a selective blocker of mechanosensitive Piezo channels, was used for better understanding of mechanotransduction in HFUMS on cells.

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KW - High-frequency ultrasound microbeam stimulation

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KW - Piezo

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DO - 10.1109/ULTSYM.2017.8091686

M3 - Conference article published in proceeding or book

BT - 2017 IEEE International Ultrasonics Symposium, IUS 2017

PB - IEEE Computer Society

T2 - 2017 IEEE International Ultrasonics Symposium, IUS 2017

Y2 - 6 September 2017 through 9 September 2017

ER -

Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

More information

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