Calcium fluorescence response of human breast cancer cells by 50-MHz ultrasound microbeam stimulation

Luchao Qi, Qi Zhang, Kwok Ho Lam, Ruibiao Guo, Runkang Chen, Jiangbang Huang, Ruoyan Meng, Zhaoyang Wang, Hairong Zheng, Ming Qian

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review


Invasiveness research is an essential step in breast cancer metastasis. The application of high-frequency ultrasound microbeam stimulation (HFUMS) offers a manner of determining the invasion potential of human breast cancer cells by eliciting the elevation of transient cytoplasmic calcium ions (Ca2+). The fluorescent change in invasive breast cancer cells (MDA-MB-231), reflecting Ca2+elevation elicited by HFUMS, is greater compared to that in weakly invasive breast cancer cells (MCF-7). GsMTx4, a selective blocker of mechanosensitive Piezo channels, was used for better understanding of mechanotransduction in HFUMS on cells.
Original languageEnglish
Title of host publication2017 IEEE International Ultrasonics Symposium, IUS 2017
PublisherIEEE Computer Society
ISBN (Electronic)9781538633830
Publication statusPublished - 31 Oct 2017
Event2017 IEEE International Ultrasonics Symposium, IUS 2017 - Washington, United States
Duration: 6 Sep 20179 Sep 2017


Conference2017 IEEE International Ultrasonics Symposium, IUS 2017
Country/TerritoryUnited States


  • Calcium fluorescence imaging
  • High-frequency ultrasound microbeam stimulation
  • Human breast cells
  • Piezo

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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