Abstract
The retinoic acid-induced differentiation of human leukemia HL-60 cells towards mature granulocytic cells was accompanied by the decline in the protein levels of c-myc, nucleophosmin/B23 and its promoter activity. These RA-induced effects were further enhanced by the concurrent treatment of HL-60 cells with p38 map kinase inhibitor SB203580 (SB). It seems that there is a strong correlation of nucleophosmin/B23 and c-Myc expressions in cells under RA treatment. Furthermore, nucleophosmin/B23 promoter activity decreased upon c-Myc antisense-mediated reduction of intracellular amount of c-Myc. CHIP assays showed that binding of c-Myc to the nucleophosmin/B23 promoter decreased in RA-treated cells. Thus, nucleophosmin/B23 expression is targeted by c-Myc during RA-induced differentiation. These results provide evidence for a novel mechanism of transcriptional downregulation of nucleophosmin/B23 and the functional role of c-Myc in RA-induced differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 211-216 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 578 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 17 Dec 2004 |
| Externally published | Yes |
Keywords
- c-Myc
- Differentiation
- Nucleophosmin/B23
- Retinoic acid
- Transcriptional regulation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology