C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

Song Lin Chua, Krishnakumar Sivakumar, Morten Rybtke, Mingjun Yuan, Jens Bo Andersen, Thomas E. Nielsen, Michael Givskov, Tim Tolker-Nielsen, Bin Cao, Staffan Kjelleberg, Liang Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

56 Citations (Scopus)

Abstract

Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

Original languageEnglish
Article number10052
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 20 May 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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