TY - JOUR
T1 - Broad-Spectrum Antimicrobial Polymer with Dual Bactericidal Mechanisms Enhances Antibiotic Activity in the Treatment of Fish Infections
AU - Yu, Yue
AU - Chen, Xianhui
AU - Pu, Huangsheng
AU - Wang, Min
AU - Song, Junfeng
AU - Yang, Anming
AU - Wan, Muyang
AU - Bai, Yugang
AU - Cai, Qinyun
AU - Yuan, Junfa
AU - Zhang, Chunhui
AU - Wong, Wing Leung
AU - Feng, Xinxin
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/7/26
Y1 - 2024/7/26
N2 - Bacterial infection in aquaculture farming has been a well-known issue, and the situation is worsened by the limitations on antibiotic usage due to environmental considerations. This study reports the design and synthesis of a broad-spectrum antimicrobial polymer, PC3-8, demonstrated for the first time as an efficient antimicrobial agent against a number of common fish pathogens, including Gram-positive and Gram-negative ones. A dual-bactericidal mechanism is proposed for PC3-8, in which the cationic polymer is found to disrupt bacterial membranes and interact with genome DNA, effectively causing bacterial cell death. PC3-8 is capable of eliminating pathogens in mammalian cell cultures, preventing them from being killed by those pathogens. When PC3-8 was applied into water, it healed bacteria-infected zebrafish, outperforming common antibiotics, such as Kanamycin and Chloramphenicol. Importantly, PC3-8 possesses low drug resistance emergence and potent synergistic effects with commercial antibiotics. Therefore, it shows great effectiveness against resistant pathogens and evades any potential environmental risk related to resistance generation. The present study demonstrates that PC3-8 is a promising antimicrobial agent for treating bacterial infection related fish diseases and has potential applications in the aquaculture industry.
AB - Bacterial infection in aquaculture farming has been a well-known issue, and the situation is worsened by the limitations on antibiotic usage due to environmental considerations. This study reports the design and synthesis of a broad-spectrum antimicrobial polymer, PC3-8, demonstrated for the first time as an efficient antimicrobial agent against a number of common fish pathogens, including Gram-positive and Gram-negative ones. A dual-bactericidal mechanism is proposed for PC3-8, in which the cationic polymer is found to disrupt bacterial membranes and interact with genome DNA, effectively causing bacterial cell death. PC3-8 is capable of eliminating pathogens in mammalian cell cultures, preventing them from being killed by those pathogens. When PC3-8 was applied into water, it healed bacteria-infected zebrafish, outperforming common antibiotics, such as Kanamycin and Chloramphenicol. Importantly, PC3-8 possesses low drug resistance emergence and potent synergistic effects with commercial antibiotics. Therefore, it shows great effectiveness against resistant pathogens and evades any potential environmental risk related to resistance generation. The present study demonstrates that PC3-8 is a promising antimicrobial agent for treating bacterial infection related fish diseases and has potential applications in the aquaculture industry.
KW - antimicrobial mechanism
KW - antimicrobial polymers
KW - fish pathogens
KW - multiple targeting
KW - synergistic combination
UR - http://www.scopus.com/inward/record.url?scp=85198971939&partnerID=8YFLogxK
U2 - 10.1021/acsapm.4c01627
DO - 10.1021/acsapm.4c01627
M3 - Journal article
AN - SCOPUS:85198971939
SN - 2637-6105
VL - 6
SP - 8618
EP - 8628
JO - ACS Applied Polymer Materials
JF - ACS Applied Polymer Materials
IS - 14
ER -