Biosensing, cytotoxicity, and cellular uptake studies of surface-modified gold nanorods

H.J. Parab, H.M. Chen, T.-C. Lai, J.H. Huang, P.H. Chen, R.-S. Liu, M. Hsiao, C.-H. Chen, Din-ping Tsai, Y.-K. Hwu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

119 Citations (Scopus)

Abstract

Herein we report biorecognition studies of protein IgG using biocomapatible gold nanorods as molecular probes. Surface modification of cetyltrimethylammonium bromide (CTAB)-stabilized gold nanorods was carried out by using poly(styrenesulfonate) (PSS) to reduce the toxicity of as-synthesized gold nanorods caused by free CTAB.?potential analysis confirmed charge reversal on the surface of gold nanorods caused by the PSS coating. Surface plasmon resonance exhibited by gold nanorods has been employed as a tool for analyzing the binding events for biomolecules. TEM results, showing the aggregation of gold nanorods, in addition to the shift in surface plasmon resonance peak in UV-vis absorption measurements, upon the interaction of biomolecules with gold nanorods, confirmed molecular binding. Morphological changes caused by the cellular uptake of gold nanorods before and after PSS modification have been observed. Cell viability studies using gold nanorods were performed to study the cytotoxic effects of these molecular probes. © 2009 American Chemical Society.
Original languageEnglish
Pages (from-to)7574-7578
Number of pages5
JournalJournal of Physical Chemistry C
Volume113
Issue number18
DOIs
Publication statusPublished - 7 May 2009
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Energy(all)
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films

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