TY - JOUR
T1 - Biodegradation of tricresyl phosphates isomers by a novel microbial consortium and the toxicity evaluation of its major products
AU - Yu, Yuanyuan
AU - Mo, Wentao
AU - Zhu, Xifen
AU - Yu, Xiaolong
AU - Sun, Jianteng
AU - Deng, Fucai
AU - Jin, Ling
AU - Yin, Hua
AU - Zhu, Lizhong
N1 - Funding Information:
This work was jointly supported by the National Key Research and Development Program of China ( 2017YFA0207003 , 2018YFC1800704 ), National Natural Science Foundations of China ( 220760314 , 2107298 ), GuangDong Basic and Applied Basic Research Foundation ( 2021A1515010366 ), Key-Area Research and Development Program of Guangdong Province ( 2019B110207002 ) and the Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education .
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - A novel microbial consortium ZY1 capable of degrading tricresyl phosphates (TCPs) was isolated, it could quickly degrade 100% of 1 mg/L tri-o-cresyl phosphate (ToCP), tri-p-cresyl phosphate (TpCP) and tri-m-cresyl phosphate (TmCP) within 36, 24 and 12 h separately and intracellular enzymes occupied the dominated role in TCPs biodegradation. Additionally, triphenyl phosphate (TPHP), 2-ethylhexyl diphenyl phosphate (EHDPP), bisphenol-A bis (diphenyl phosphate) (BDP), tris (2-chloroethyl) phosphate (TCEP) and tris (1-chloro-2-propyl) phosphate (TCPP) could also be degraded by ZY1 and the aryl-phosphates was easier to be degraded. The TCPs reduction observed in freshwater and seawater indicated that high salinity might weak the degradability of ZY1. The detected degradation products suggested that TCPs was mainly metabolized though the hydrolysis and hydroxylation. Sequencing analysis presented that the degradation of TCPs relied on the cooperation between sphingobacterium, variovorax and flavobacterium. The cytochrome P450/NADPH-cytochrome P450 reductase and phosphatase were speculated might involve in TCPs degradation. Finally, toxicity evaluation study found that the toxicity of the diesters products was lower than their parent compound based on the generation of the intracellular reactive oxygen (ROS) and the apoptosis rate of A549 cell. Taken together, this research provided a new insight for the bioremediation of TCPs in actual environment.
AB - A novel microbial consortium ZY1 capable of degrading tricresyl phosphates (TCPs) was isolated, it could quickly degrade 100% of 1 mg/L tri-o-cresyl phosphate (ToCP), tri-p-cresyl phosphate (TpCP) and tri-m-cresyl phosphate (TmCP) within 36, 24 and 12 h separately and intracellular enzymes occupied the dominated role in TCPs biodegradation. Additionally, triphenyl phosphate (TPHP), 2-ethylhexyl diphenyl phosphate (EHDPP), bisphenol-A bis (diphenyl phosphate) (BDP), tris (2-chloroethyl) phosphate (TCEP) and tris (1-chloro-2-propyl) phosphate (TCPP) could also be degraded by ZY1 and the aryl-phosphates was easier to be degraded. The TCPs reduction observed in freshwater and seawater indicated that high salinity might weak the degradability of ZY1. The detected degradation products suggested that TCPs was mainly metabolized though the hydrolysis and hydroxylation. Sequencing analysis presented that the degradation of TCPs relied on the cooperation between sphingobacterium, variovorax and flavobacterium. The cytochrome P450/NADPH-cytochrome P450 reductase and phosphatase were speculated might involve in TCPs degradation. Finally, toxicity evaluation study found that the toxicity of the diesters products was lower than their parent compound based on the generation of the intracellular reactive oxygen (ROS) and the apoptosis rate of A549 cell. Taken together, this research provided a new insight for the bioremediation of TCPs in actual environment.
KW - Bioaugmentation
KW - Metabolic pathways
KW - Microbial community
KW - Toxicity
KW - Tricresyl phosphates
UR - http://www.scopus.com/inward/record.url?scp=85126327001&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2022.154415
DO - 10.1016/j.scitotenv.2022.154415
M3 - Journal article
SN - 0048-9697
VL - 828
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 154415
ER -