Biodegradable nanocapsules as siRNA carriers for mutant k-ras gene silencing of human pancreatic carcinoma cells

Guimiao Lin, Rui Hu, Wing Cheung Law, Chih Kuang Chen, Yucheng Wang, Hui Li Chin, Quoc Toan Nguyen, Cheng Kee Lai, Ho Sup Yoon, Xiaomei Wang, Gaixia Xu, Ling Ye, Chong Cheng, Ken Tye Yong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

30 Citations (Scopus)

Abstract

The application of small interfering RNA (siRNA)-based RNA interference (RNAi) for cancer gene therapy has attracted great attention. Gene therapy is a promising strategy for cancer treatment because it is relatively non-invasive and has a higher therapeutic specificity than chemotherapy. However, without the use of safe and efficient carriers, siRNAs cannot effectively penetrate the cell membranes and RNAi is impeded. In this work, cationic poly(lactic acid) (CPLA)-based degradable nanocapsules (NCs) are utilized as novel carriers of siRNA for effective gene silencing of pancreatic cancer cells. These CPLA-NCs can readily form nanoplexes with K-Ras siRNA and over 90% transfection efficiency is achieved using the nanoplexes. Cell viability studies show that the nanoparticles are highly biocompatible and non-toxic, indicating that CPLA-NC is a promising potential candidate for gene therapy in a clinical setting. Cationic poly(lactic acid) (CPLA)-based degradable nanocapsules (NCs) are utilized as novel carriers of siRNA for effective gene silencing of pancreatic cancer cells. These CPLA-NCs can readily form nanoplexes with K-Ras siRNA and over 90% transfection efficiency is achieved using the nanoplexes.
Original languageEnglish
Pages (from-to)2757-2763
Number of pages7
JournalSmall
Volume9
Issue number16
DOIs
Publication statusPublished - 26 Aug 2013
Externally publishedYes

Keywords

  • biodegradable
  • gene therapy
  • pancreatic cancer
  • polymer nanocapsules
  • siRNA

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

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