Biodegradable cationic polymeric nanocapsules for overcoming multidrug resistance and enabling drug-gene co-delivery to cancer cells

Chih Kuang Chen, Wing Cheung Law, Ravikumar Aalinkeel, Yun Yu, Bindukumar Nair, Jincheng Wu, Supriya Mahajan, Jessica L. Reynolds, Yukun Li, Cheng Kee Lai, Emmanuel S. Tzanakakis, Stanley A. Schwartz, Paras N. Prasad, Chong Cheng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

88 Citations (Scopus)

Abstract

Having unique architectural features, cationic polymeric nanocapsules (NCs) with well-defined covalently stabilized biodegradable structures were generated as potentially universal and safe therapeutic nanocarriers. These NCs were synthesized from allyl-functionalized cationic polylactide (CPLA) by highly efficient UV-induced thiol-ene interfacial cross-linking in transparent miniemulsions. With tunable nanoscopic sizes, negligible cytotoxicity and remarkable degradability, they are able to encapsulate doxorubicin (Dox) with inner cavities and bind interleukin-8 (IL-8) small interfering RNA (siRNA) with cationic shells. The Dox-encapsulated NCs can effectively bypass the P-glycoprotein (Pgp)-mediated multidrug resistance of MCF7/ADR cancer cells, thereby resulting in increased intracellular drug concentration and reduced cell viability. In vitro studies also showed that the NCs loaded with Dox, IL-8 siRNA and both agents can be readily taken up by PC3 prostate cancer cells, resulting in a significant chemotherapeutic effect and/or IL-8 gene silencing.
Original languageEnglish
Pages (from-to)1567-1572
Number of pages6
JournalNanoscale
Volume6
Issue number3
DOIs
Publication statusPublished - 7 Feb 2014
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)

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