Bioconversion of beetle-killed lodgepole pine using SPORL: Process scale-up design, lignin coproduct, and high solids fermentation without detoxification

Haifeng Zhou, J. Y. Zhu, Xiaolin Luo, Shao Yuan Leu, Xiaolei Wu, Roland Gleisner, Bruce S. Dien, Ronald E. Hector, Dongjie Yang, Xueqing Qiu, Eric Horn, Jose Negron

Research output: Journal article publicationJournal articleAcademic researchpeer-review

59 Citations (Scopus)

Abstract

Mountain pine beetle killed Lodgepole pine (Pinus contorta Douglas ex Loudon) wood chips were pretreated using an acidic sulfite solution of approximately pH = 2.0 at a liquor to wood ratio of 3 and sodium bisulfite loading of 8 wt % on wood. The combined hydrolysis factor (CHF), formulated from reaction kinetics, was used to design a scale-up pretreatment on 2000 g wood chips at a relatively low temperature of 165 C that reduced furan formation and facilitated high solids saccharification and fermentation. The pretreated solids and liquor were disk milled together to result in a biomass whole slurry of 25% total solids. The whole biomass slurry was directly used to conduct simultaneous enzymatic saccharification and combined fermentation (SSCombF) using a commercial cellulase and Saccharomyces cerevisiae YRH400 without detoxification. A terminal ethanol titer of 47.1 g L-1with a yield of 306 L (tonne wood)-1, or 72.0% theoretical, was achieved when SSCombF was conducted at an unwashed solids loading of 18%. The lignosulfonate (LS) from SPORL was highly sulfonated and showed better dispersibility than a high purity commercial softwood LS, and therefore has potential as a directly marketable coproduct.
Original languageEnglish
Pages (from-to)16057-16065
Number of pages9
JournalIndustrial and Engineering Chemistry Research
Volume52
Issue number45
DOIs
Publication statusPublished - 13 Nov 2013

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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