Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: In vitro and in vivo studies

Liwei Liu; Ken Tye Yong; Indrajit Roy; Wing Cheung Law; Ling Ye; Jianwei Liu; Jing Liu; Rajiv Kumar; Xihe Zhang; Paras N. Prasad

doi:10.7150/thno.3456

Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: In vitro and in vivo studies

Liwei Liu, Ken Tye Yong, Indrajit Roy, Wing Cheung Law, Ling Ye, Jianwei Liu, Jing Liu, Rajiv Kumar, Xihe Zhang, Paras N. Prasad

Research output: Journal article publication › Journal article › Academic research › peer-review

72 Citations (Scopus)

Abstract

Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible in vitro toxicity. The in vitro study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. In vivo imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.

Original language	English
Pages (from-to)	705-713
Number of pages	9
Journal	Theranostics
Volume	2
Issue number	7
DOIs	https://doi.org/10.7150/thno.3456
Publication status	Published - 14 Sept 2012
Externally published	Yes

Keywords

Bioconjugation
Bioimaging
Pluronics and Nanotoxicity
Quantum dots
Targeted delivery

ASJC Scopus subject areas

Medicine (miscellaneous)
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.7150/thno.3456

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title = "Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: In vitro and in vivo studies",

abstract = "Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible in vitro toxicity. The in vitro study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. In vivo imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.",

keywords = "Bioconjugation, Bioimaging, Pluronics and Nanotoxicity, Quantum dots, Targeted delivery",

author = "Liwei Liu and Yong, {Ken Tye} and Indrajit Roy and Law, {Wing Cheung} and Ling Ye and Jianwei Liu and Jing Liu and Rajiv Kumar and Xihe Zhang and Prasad, {Paras N.}",

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doi = "10.7150/thno.3456",

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AU - Liu, Liwei

AU - Yong, Ken Tye

AU - Roy, Indrajit

AU - Law, Wing Cheung

AU - Ye, Ling

AU - Liu, Jianwei

AU - Liu, Jing

AU - Kumar, Rajiv

AU - Zhang, Xihe

AU - Prasad, Paras N.

PY - 2012/9/14

Y1 - 2012/9/14

N2 - Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible in vitro toxicity. The in vitro study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. In vivo imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.

AB - Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible in vitro toxicity. The in vitro study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. In vivo imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.

KW - Bioconjugation

KW - Bioimaging

KW - Pluronics and Nanotoxicity

KW - Quantum dots

KW - Targeted delivery

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DO - 10.7150/thno.3456

M3 - Journal article

SN - 1838-7640

VL - 2

SP - 705

EP - 713

JO - Theranostics

JF - Theranostics

IS - 7

ER -

Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: In vitro and in vivo studies

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

More information

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