Biochemical and Microstructural Characteristics of Insoluble and Soluble Dietary Fiber Prepared from Mushroom Sclerotia of Pleurotus tuber-regium, Polyporus rhinocerus, and Wolfiporia cocos

Ka Hing Wong, Peter C.K. Cheung, Jin Zhong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

33 Citations (Scopus)

Abstract

The proximate composition of sclerotia of Pleurotus tuber-regium, Polyporus rhinoceros, and Wolfiporia cocos, together with the yield, purity, monosaccharide profile, and microstructure of their insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) fractions prepared from AOAC enzymatic-gravimetric methods were investigated and compared. All three scierotia were typical carbohydrate rich sclerotia [ranging from 90.5 to 98.1% dry matter (DM)] with an exceptionally low amount of crude lipid content (ranging from 0.02 to 0.14% DM). Besides, all three sclerotia possessed substantial amounts of IDF (ranging from 77.4 to 94.6% DM) with notably high levels of nonstarch polysaccharides (NSP) (89.9-92.2% DM) in which glucose was the predominant sugar residue (90.6-97.2% of NSP DM). On the contrary, both the yield (only ranging from 1.45 to 2.50% DM) and the amount of NSP (ranging from 22.4 to 29.6% DM) of the three sclerotial SDF fractions were very low. Scanning electron micrographs showed fragments of interwoven hyphae and insoluble materials in the three sclerotial IDF fractions, but only an amorphous structure of soluble materials was observed in the SDF fractions. The potential use of these fiber preparations was discussed.
Original languageEnglish
Pages (from-to)7197-7202
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number24
DOIs
Publication statusPublished - 19 Nov 2003
Externally publishedYes

Keywords

  • IDF
  • Microstructure
  • Monosaccharide profile
  • Mushroom sclerotia
  • P. rhinoceros
  • P. tuber-regium
  • SDF
  • W. cocos

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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