TY - JOUR
T1 - Bioavailability-based assessment of aryl hydrocarbon receptor-mediated activity in Lake Tai Basin from Eastern China
AU - Li, Juan Ying
AU - Su, Lei
AU - Wei, Fenghua
AU - Yang, Jianghua
AU - Jin, Ling
AU - Zhang, Xiaowei
N1 - Funding Information:
The present research was supported by Shanghai Universities First-class Disciplines Project of Fisheries and Shanghai Education Commission Innovation Project ( 2015Z10264003 ) and Jiangsu Provincial Key Technology R&D Program (# BE2011776 ).
Publisher Copyright:
© 2015 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/2/15
Y1 - 2016/2/15
N2 - Coupling polydimethylsiloxane (PDMS)-based equilibrium passive sampling with chemical and bioassay analysis, we assessed aryl hydrocarbon receptor (AhR)-mediated activity and contributing chemicals in sediment from Lake Tai Basin, Eastern China. The bioanalytical equivalent concentrations (BEQs) of AhR-active chemicals for the exhaustive (total burden) and PDMS extracts (bioavailable fractions) ranged from <9.5-300ng TCDD-EQ/kgdry weight (dw) and <0.096-2.2ng TCDD-EQ/kgdw, respectively, which were of average levels compared to those reported elsewhere. The total concentrations of PAHs in sediment and PDMS were 17-4700μg/kgdw and 0.61-10μg/kgdw, respectively. The majority of the exhaustive extracts subject to acid treatment showed >70% decline in AhR-mediated activity, suggesting the minor contribution by persistent AhR ligands. Targeted analysis of polycyclic aromatic hydrocarbons (PAHs) showed, however, that these chemicals contributed <40% to the overall effect in both exhaustive and PDMS extracts, indicating the presence of other labile AhR ligands. The concentrations of PAHs and BEQs of the AhR-mediated activity attributed to these chemicals in the exhaustive extracts can be back calculated from those in the PDMS extracts via a general organic carbon-PDMS partition coefficient. Similar quantitative conversion between PDMS and aquatic organisms was also verified for aquatic organisms via the lipid-PDMS partition coefficient. Therefore, our study provided a first insight into the quantitative links between bulk chemical burdens in sediment, chemical bioavailability, bioaccumulation potential and resulting mixture effects, as an integral part of predictive environmental risk assessment of contaminated sediment.
AB - Coupling polydimethylsiloxane (PDMS)-based equilibrium passive sampling with chemical and bioassay analysis, we assessed aryl hydrocarbon receptor (AhR)-mediated activity and contributing chemicals in sediment from Lake Tai Basin, Eastern China. The bioanalytical equivalent concentrations (BEQs) of AhR-active chemicals for the exhaustive (total burden) and PDMS extracts (bioavailable fractions) ranged from <9.5-300ng TCDD-EQ/kgdry weight (dw) and <0.096-2.2ng TCDD-EQ/kgdw, respectively, which were of average levels compared to those reported elsewhere. The total concentrations of PAHs in sediment and PDMS were 17-4700μg/kgdw and 0.61-10μg/kgdw, respectively. The majority of the exhaustive extracts subject to acid treatment showed >70% decline in AhR-mediated activity, suggesting the minor contribution by persistent AhR ligands. Targeted analysis of polycyclic aromatic hydrocarbons (PAHs) showed, however, that these chemicals contributed <40% to the overall effect in both exhaustive and PDMS extracts, indicating the presence of other labile AhR ligands. The concentrations of PAHs and BEQs of the AhR-mediated activity attributed to these chemicals in the exhaustive extracts can be back calculated from those in the PDMS extracts via a general organic carbon-PDMS partition coefficient. Similar quantitative conversion between PDMS and aquatic organisms was also verified for aquatic organisms via the lipid-PDMS partition coefficient. Therefore, our study provided a first insight into the quantitative links between bulk chemical burdens in sediment, chemical bioavailability, bioaccumulation potential and resulting mixture effects, as an integral part of predictive environmental risk assessment of contaminated sediment.
KW - Bioavailability
KW - In vitro bioassay
KW - Lake Tai Basin
KW - Mixture effect
KW - Passive sampling
KW - Polycyclic aromatic hydrocarbon
UR - http://www.scopus.com/inward/record.url?scp=84954193543&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2015.12.041
DO - 10.1016/j.scitotenv.2015.12.041
M3 - Journal article
C2 - 26706770
AN - SCOPUS:84954193543
VL - 544
SP - 987
EP - 994
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
ER -