Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform

Kin Fong Lei; Wing Cheung Law; Yick Keung Suen; Wen J. Li; Ho Pui Ho; Chinlon Lin; Siu Kai Kong

doi:10.1109/NANO.2005.1500764

Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform

Kin Fong Lei
, Wing Cheung Law
, Yick Keung Suen
, Wen J. Li
, Ho Pui Ho
, Chinlon Lin
, Siu Kai Kong

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

1 Citation (Scopus)

Abstract

This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and a SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. Because SPR based biodetection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass microfluidic systems, if bio-compatibility and low-cost are desired. The integrated system has been studied for its potential applications in bio-molecules detection and drug-discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show its biomedical applications. The experimental setup and results of these tests are presented in this paper.

Original language	English
Title of host publication	2005 5th IEEE Conference on Nanotechnology
Pages	515-518
Number of pages	4
Volume	1
DOIs	https://doi.org/10.1109/NANO.2005.1500764
Publication status	Published - 1 Dec 2005
Externally published	Yes
Event	2005 5th IEEE Conference on Nanotechnology - Nagoya, Japan Duration: 11 Jul 2005 → 15 Jul 2005

Conference

Conference	2005 5th IEEE Conference on Nanotechnology
Country/Territory	Japan
City	Nagoya
Period	11/07/05 → 15/07/05

Keywords

Biomolecular Detection
Cellular Detection
Microfluidic System
SPR Sensor

ASJC Scopus subject areas

General Engineering

More information

10.1109/NANO.2005.1500764

Cite this

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title = "Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform",

abstract = "This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and a SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. Because SPR based biodetection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass microfluidic systems, if bio-compatibility and low-cost are desired. The integrated system has been studied for its potential applications in bio-molecules detection and drug-discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show its biomedical applications. The experimental setup and results of these tests are presented in this paper.",

keywords = "Biomolecular Detection, Cellular Detection, Microfluidic System, SPR Sensor",

author = "Lei, \{Kin Fong\} and Law, \{Wing Cheung\} and Suen, \{Yick Keung\} and Li, \{Wen J.\} and Ho, \{Ho Pui\} and Chinlon Lin and Kong, \{Siu Kai\}",

year = "2005",

month = dec,

day = "1",

doi = "10.1109/NANO.2005.1500764",

language = "English",

isbn = "0780391993",

volume = "1",

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booktitle = "2005 5th IEEE Conference on Nanotechnology",

note = "2005 5th IEEE Conference on Nanotechnology ; Conference date: 11-07-2005 Through 15-07-2005",

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Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform. / Lei, Kin Fong; Law, Wing Cheung; Suen, Yick Keung et al.
2005 5th IEEE Conference on Nanotechnology. Vol. 1 2005. p. 515-518 1500764.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform

AU - Lei, Kin Fong

AU - Law, Wing Cheung

AU - Suen, Yick Keung

AU - Li, Wen J.

AU - Ho, Ho Pui

AU - Lin, Chinlon

AU - Kong, Siu Kai

PY - 2005/12/1

Y1 - 2005/12/1

N2 - This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and a SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. Because SPR based biodetection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass microfluidic systems, if bio-compatibility and low-cost are desired. The integrated system has been studied for its potential applications in bio-molecules detection and drug-discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show its biomedical applications. The experimental setup and results of these tests are presented in this paper.

AB - This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and a SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. Because SPR based biodetection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass microfluidic systems, if bio-compatibility and low-cost are desired. The integrated system has been studied for its potential applications in bio-molecules detection and drug-discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show its biomedical applications. The experimental setup and results of these tests are presented in this paper.

KW - Biomolecular Detection

KW - Cellular Detection

KW - Microfluidic System

KW - SPR Sensor

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U2 - 10.1109/NANO.2005.1500764

DO - 10.1109/NANO.2005.1500764

M3 - Conference article published in proceeding or book

SN - 0780391993

SN - 9780780391994

VL - 1

SP - 515

EP - 518

BT - 2005 5th IEEE Conference on Nanotechnology

T2 - 2005 5th IEEE Conference on Nanotechnology

Y2 - 11 July 2005 through 15 July 2005

ER -

Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform

Abstract

Conference

Keywords

ASJC Scopus subject areas

More information

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