Bio-molecular and cellular detection using SPR sensor and all-transparent microfluidic platform

Kin Fong Lei, Wing Cheung Law, Yick Keung Suen, Wen J. Li, Ho Pui Ho, Chinlon Lin, Siu Kai Kong

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

1 Citation (Scopus)

Abstract

This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and a SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. Because SPR based biodetection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass microfluidic systems, if bio-compatibility and low-cost are desired. The integrated system has been studied for its potential applications in bio-molecules detection and drug-discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show its biomedical applications. The experimental setup and results of these tests are presented in this paper.
Original languageEnglish
Title of host publication2005 5th IEEE Conference on Nanotechnology
Pages515-518
Number of pages4
Volume1
DOIs
Publication statusPublished - 1 Dec 2005
Externally publishedYes
Event2005 5th IEEE Conference on Nanotechnology - Nagoya, Japan
Duration: 11 Jul 200515 Jul 2005

Conference

Conference2005 5th IEEE Conference on Nanotechnology
CountryJapan
CityNagoya
Period11/07/0515/07/05

Keywords

  • Biomolecular Detection
  • Cellular Detection
  • Microfluidic System
  • SPR Sensor

ASJC Scopus subject areas

  • Engineering(all)

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