Behavioral and neurochemical characterization of transgenic mice carrying the human presenilin-1 gene with or without the leucine-to-proline mutation at codon 235

X. G. Huang, Kay Yan Benjamin Yee, S. Nag, S. T.H. Chan, F. Tang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

28 Citations (Scopus)

Abstract

Human presenilin-1 (PS1) mutations are associated with the incidence of familial Alzheimer's disease. The present study evaluated the behavioral and neurochemical effects of the L235P mutation (substitution of leucine by proline at codon 235) of the human PS1 gene, which has been linked to a form of early-onset Alzheimer's disease. Except for a significant increase in the production of β-amyloid-42, the mutant mice did not show any overt signs of Alzheimer-like neuropathology in the form of plaque formation, changes in choline acetyltransferase activity, or somatostatin content in the brain. Cognitive assays indicated that the mutation did not affect the acquisition or reversal of a spatial reference memory task in the water maze or performance on a spatial working memory task. In contrast, L235P PS1 transgenic mice exhibited a significant impairment in a test of spontaneous object recognition. This dissociation is suggestive of a preferential impairment of the extrahippocampal memory system and is consistent with what has been reported in another pathological mutation (substitution of leucine by valine at codon 286) of the PS1 gene.
Original languageEnglish
Pages (from-to)673-681
Number of pages9
JournalExperimental Neurology
Volume183
Issue number2
DOIs
Publication statusPublished - 1 Oct 2003
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Choline acetyltransferase
  • Object recognition memory
  • Presenilin-1
  • Somatostatin
  • Water maze
  • β-Amyloid

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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