Autophagy is an important action mode for functionalized selenium nanoparticles to exhibit anti-colorectal cancer activity

Guanning Huang, Zumei Liu, Lizhen He, Kar Him Luk, Siu To Cheung, Ka Hing Wong (Corresponding Author), Tianfeng Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

29 Citations (Scopus)

Abstract

Selenium nanoparticles (SeNPs) have attracted much interest as potential anticancer nanodrugs. Our previous studies also demonstrated that SeNPs could be developed as carriers of clinically used anticancer drugs to achieve synergistic efficacy. Here, we describe the synthesis of Pleurotus tuber-regium (PTR)-conjugated SeNPs (PTR-SeNPs) and their application in the treatment of colorectal cancer (CRC), which is one of the principal causes of cancer morbidity and mortality in the world. PTR-SeNPs were absorbed by cancer cells via clathrin-mediated endocytosis into lysosomes and caveolae-mediated endocytosis into the Golgi apparatus. Internalized PTR-SeNPs trigger intracellular dose- and time-dependent G2/M phase arrest and apoptosis. Moreover, as shown by using a pEGFP-LC3 plasmid transfection model, PTR-SeNPs activate autophagy to promote the death of cancer cells via upregulation of beclin 1-related signaling pathways. In summary, this study demonstrates the high efficacy of functionalized SeNPs for therapy of colorectal cancer and reveals the important role of autophagy in promoting apoptosis and cell cycle arrest to induce cell death.

Original languageEnglish
Pages (from-to)2508-2517
Number of pages10
JournalBiomaterials Science
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 2018

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)

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