Automated Single-microparticle Patterning System for Micro-analytics

Kaicheng Huang, Zhenxi Cui, Ihab Abu Ajamieh, Jiewen Lai, James K. Mills, Henry K. Chu

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

Micropatterning has been applied in pharmaceutical research and drug discovery as an effective tool. Assays and tests can be easily performed by arranging microparticles in an array. However, the quality of the microparticle pattern influences the reliability of the results. In this study, an automatic single-microparticle patterning system was developed. This system enables precise patterning of single microparticles through dielectrophoresis, which can manipulate micro-objects (e.g., bead, protein, and cell). Orange fluorescent polystyrene beads (40 μ m) were suspended in 6-aminohexanoic acid solution. In contrast to the conventional microfluidic configuration, electrode-based microchip suspended above the substrate can selectively trap and pattern the microbeads. In particular, the microbeads laying on the substrate can be displaced to different positions relative to the patterning electrodes in the microchip. A vision-based approach was used to evaluate necessary information such as the gap distance and positions of the electrodes and microbeads in the image. Experiments were performed to examine the strategy used to construct high-quality single-bead patterns. With the proposed system, different single microbead patterns can be successfully constructed on a glass substrate. Results confirmed that this system offers an automatic method with high flexibility to construct different single microparticle patterns for various applications.

Original languageEnglish
Title of host publication2020 IEEE 16th International Conference on Automation Science and Engineering, CASE 2020
PublisherIEEE Computer Society
Pages390-396
Number of pages7
ISBN (Electronic)9781728169040
DOIs
Publication statusPublished - Aug 2020
Event16th IEEE International Conference on Automation Science and Engineering, CASE 2020 - Hong Kong, Hong Kong
Duration: 20 Aug 202021 Aug 2020

Publication series

NameIEEE International Conference on Automation Science and Engineering
Volume2020-August
ISSN (Print)2161-8070
ISSN (Electronic)2161-8089

Conference

Conference16th IEEE International Conference on Automation Science and Engineering, CASE 2020
Country/TerritoryHong Kong
CityHong Kong
Period20/08/2021/08/20

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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