TY - GEN
T1 - Automated cell patterning system with a microchip using dielectrophoresis
AU - Huang, Kaicheng
AU - Chu, Henry K.
AU - Lu, Bo
AU - Lai, Jiewen
AU - Cheng, Li
PY - 2019/5
Y1 - 2019/5
N2 - The ability to patterning cells is an important technique to facilitate cell-based assay and characterization. In this paper, an automated cell patterning system was developed for the fabrication of large-scale cell patterns. To resolve the challenge of the limited printable area, the cell-printing microchip and the substrate were mounted on the movable stages of the system, and large-scale cell patterns were realized through coordination between the stages. An autofocusing technique was integrated in the system to evaluate the gap between the microchip and the substrate. In order to enhance the performance of the patterning system, different experimental parameters, including the velocity of the moving stage, were examined. Yeast cells suspending in 6-aminohexanoic acid (AHA) solution were considered in this study, and a sequence of characters was successfully printed using the proposed system. The results confirm that this system offers an automatic method with high flexibility to construct large-scale cell patterns for various applications.
AB - The ability to patterning cells is an important technique to facilitate cell-based assay and characterization. In this paper, an automated cell patterning system was developed for the fabrication of large-scale cell patterns. To resolve the challenge of the limited printable area, the cell-printing microchip and the substrate were mounted on the movable stages of the system, and large-scale cell patterns were realized through coordination between the stages. An autofocusing technique was integrated in the system to evaluate the gap between the microchip and the substrate. In order to enhance the performance of the patterning system, different experimental parameters, including the velocity of the moving stage, were examined. Yeast cells suspending in 6-aminohexanoic acid (AHA) solution were considered in this study, and a sequence of characters was successfully printed using the proposed system. The results confirm that this system offers an automatic method with high flexibility to construct large-scale cell patterns for various applications.
UR - http://www.scopus.com/inward/record.url?scp=85071483392&partnerID=8YFLogxK
U2 - 10.1109/ICRA.2019.8794177
DO - 10.1109/ICRA.2019.8794177
M3 - Conference article published in proceeding or book
AN - SCOPUS:85071483392
T3 - Proceedings - IEEE International Conference on Robotics and Automation
SP - 634
EP - 639
BT - 2019 International Conference on Robotics and Automation, ICRA 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 International Conference on Robotics and Automation, ICRA 2019
Y2 - 20 May 2019 through 24 May 2019
ER -