AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

Chi Shing Tai, David K. Banfield

Research output: Journal article publicationJournal articleAcademic researchpeer-review

18 Citations (Scopus)

Abstract

SNAREs are membrane-associated proteins that play a central role in vesicle targeting and intra-cellular membrane fusion reactions in eukaryotic cells. Here we describe the identification of AtBS14a and AtBS14b, putative SNAREs from Arabidopsis thaliana that share 60% amino acid sequence identity. Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive growth defect in sft1-1 cells and over-expression of either AtBS14a or AtBS14b can support the growth of sft1Δ cells but not bet1Δ cells. These data together with structure-function and biochemical studies presented herein suggest that AtBS14a and AtBS14b share properties that are consistent with them being members of the Bet1/Sft1 SNARE protein family.
Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalFEBS Letters
Volume500
Issue number3
DOIs
Publication statusPublished - 6 Jul 2001
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • BET1
  • Functional complementation
  • SFT1
  • SNARE

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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