Assessment of various carbon sources and nutrient feeding strategies for Panax ginseng cell culture

Jianyong Wu, Kwok Ping Ho

Research output: Journal article publicationJournal articleAcademic researchpeer-review

18 Citations (Scopus)

Abstract

Ginseng (root of Panax ginseng C. A. Meyer) cells were cultivated on medium supplemented with various carbohydrates including sucrose, glucose, and fructose, at initial concentrations ranging from 10 to 110 g/L. Sucrose was shown to be the superior carbon source to the monosaccharides for ginseng cell growth and the optimal concentration was between 30 and 50 g/L. An increase in the initial concentration within this range increased the maximum cell density and growth index significantly, whereas much higher concentrations inhibited cell growth. Feeding of sucrose and some other medium components during the growth (fed-batch mode) was more effective in enhancing the cell growth and biomass productivity, increasing the growth index by more than 60-70% and biomass productivity by more than 50%.
Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume82
Issue number1
DOIs
Publication statusPublished - 1 Jan 1999

Keywords

  • Biomass productivity
  • Carbon source
  • Nutrient feeding
  • Panax ginseng
  • Plant cell culture
  • Sugar concentration

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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