Application of organic nonsolvent in the process of solution-enhanced dispersion by supercritical CO2 to prepare puerarin fine particles

Ai Zheng Chen, Yi Li, Foo Tim Chau, Tsui Yan Lau, Jun Yan Hu, Zheng Zhao, Kam Wah Mok

Research output: Journal article publicationJournal articleAcademic researchpeer-review

59 Citations (Scopus)


An organic nonsolvent, dichloromethane was employed in the process of solution-enhanced dispersion by supercritical CO2(SEDS) to prepare fine particles of puerarin. A 23factorial experiment was designed to investigate and identify the relative significance of the processing parameters on the surface morphology, particle size and particle size distribution of the products. The effect of the nonsolvent/solvent ratio was found to be dominant in the results regarding particle size. Increasing the nonsolvent content of the puerarin solution decreased the particle size significantly. After optimization, the resulting puerarin nanoparticles exhibited a good spherical shape, a smooth surface and a narrow particle size distribution, with a mean particle size of 0.19 μm. After SEDS processing, the measurements of high performance liquid chromatography (HPLC), ultraviolet (UV) and mass spectrometry (MS) indicated there was no change in the chemical composition of puerarin particles; Fourier transform infrared (FTIR) spectroscopy measurement found the minor structural changes occurred on a molecular level; X-ray powder diffraction (XRPD) analysis revealed that the physical state of puerarin shifted into an amorphous form; and a significant increase in the dissolution rate of puerarin nanoparticles was observed. The SEDS process combined with the addition of dichloromethane could produce puerarin nanoparticles without contamination.
Original languageEnglish
Pages (from-to)394-402
Number of pages9
JournalJournal of Supercritical Fluids
Issue number3
Publication statusPublished - 1 Jul 2009


  • Nanoparticles
  • Nonsolvent
  • Puerarin
  • Supercritical fluids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemical Engineering(all)
  • Condensed Matter Physics

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