Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors

H. P. Ho; S. Y. Wu; Wing Cheung Law; Chinlon Lin

Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors

H. P. Ho, S. Y. Wu, Wing Cheung Law, Chinlon Lin

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48 × 10-8 refractive index unit (RIU), which is three times better than that reported from conventional angular systems.

Original language	English
Title of host publication	Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004
Pages	221-222
Number of pages	2
Publication status	Published - 1 Dec 2004
Externally published	Yes
Event	Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, Taiwan Duration: 15 Dec 2004 → 17 Dec 2004

Conference

Conference	Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004
Country/Territory	Taiwan
City	Taipei
Period	15/12/04 → 17/12/04

ASJC Scopus subject areas

General Engineering

Cite this

@inproceedings{1d881a46270640549ebe1c0aa1ed4ff8,

title = "Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors",

abstract = "Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48 × 10-8 refractive index unit (RIU), which is three times better than that reported from conventional angular systems.",

author = "Ho, {H. P.} and Wu, {S. Y.} and Law, {Wing Cheung} and Chinlon Lin",

year = "2004",

month = dec,

day = "1",

language = "English",

isbn = "0780386760",

pages = "221--222",

booktitle = "Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004",

note = "Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 ; Conference date: 15-12-2004 Through 17-12-2004",

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Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors. / Ho, H. P.; Wu, S. Y.; Law, Wing Cheung et al.
Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004. 2004. p. 221-222.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors

AU - Ho, H. P.

AU - Wu, S. Y.

AU - Law, Wing Cheung

AU - Lin, Chinlon

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48 × 10-8 refractive index unit (RIU), which is three times better than that reported from conventional angular systems.

AB - Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48 × 10-8 refractive index unit (RIU), which is three times better than that reported from conventional angular systems.

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M3 - Conference article published in proceeding or book

SN - 0780386760

SP - 221

EP - 222

BT - Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004

T2 - Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004

Y2 - 15 December 2004 through 17 December 2004

ER -

Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors

Abstract

Conference

ASJC Scopus subject areas

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