Application of a novel widefield surface plasmon microscope in binding assays

S. Marlafeka, S. Liu, M. Mahadi Abdul Jamil, P. C. Twigg, S. T. Britland, M. C.T. Denyer, C. W. See, Michael Geoffrey Somekh

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

1 Citation (Scopus)

Abstract

The present work investigates the capabilities of a novel widefield surface plasmon microscope (WSPR) and its potential applications in biology. Here in particular, we concentrate on imaging interfacial interactions between specific antibodies and the proteins laminin and fibronectin, both patterned onto prefabricated Au / Cr / glass substrates. These experiments are used to demonstrate the high sensitivity, good contrast and high lateral resolution (close to a micron) of the new system. These results indicate that our microscope may form the basis for a new generation of fast assay systems.
Original languageEnglish
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages140-142
Number of pages3
Volume2005
DOIs
Publication statusPublished - 1 Dec 2005
Externally publishedYes
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: 12 May 200515 May 2005

Conference

Conference2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
CountryUnited States
CityOahu, HI
Period12/05/0515/05/05

Keywords

  • Cell
  • Immunoassay
  • Surface plasmon
  • Widefield

ASJC Scopus subject areas

  • Engineering(all)

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