Application of a novel widefield surface plasmon microscope in binding assays

S. Marlafeka; S. Liu; M. Mahadi Abdul Jamil; P. C. Twigg; S. T. Britland; M. C.T. Denyer; C. W. See; Michael Geoffrey Somekh

doi:10.1109/MMB.2005.1548406

Application of a novel widefield surface plasmon microscope in binding assays

S. Marlafeka, S. Liu, M. Mahadi Abdul Jamil, P. C. Twigg, S. T. Britland, M. C.T. Denyer, C. W. See, Michael Geoffrey Somekh

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

1 Citation (Scopus)

Abstract

The present work investigates the capabilities of a novel widefield surface plasmon microscope (WSPR) and its potential applications in biology. Here in particular, we concentrate on imaging interfacial interactions between specific antibodies and the proteins laminin and fibronectin, both patterned onto prefabricated Au / Cr / glass substrates. These experiments are used to demonstrate the high sensitivity, good contrast and high lateral resolution (close to a micron) of the new system. These results indicate that our microscope may form the basis for a new generation of fast assay systems.

Original language	English
Title of host publication	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages	140-142
Number of pages	3
Volume	2005
DOIs	https://doi.org/10.1109/MMB.2005.1548406
Publication status	Published - 1 Dec 2005
Externally published	Yes
Event	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States Duration: 12 May 2005 → 15 May 2005

Conference

Conference	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Country/Territory	United States
City	Oahu, HI
Period	12/05/05 → 15/05/05

Keywords

Cell
Immunoassay
Surface plasmon
Widefield

ASJC Scopus subject areas

General Engineering

More information

10.1109/MMB.2005.1548406

Cite this

Marlafeka, S., Liu, S., Mahadi Abdul Jamil, M., Twigg, P. C., Britland, S. T., Denyer, M. C. T., See, C. W., & Somekh, M. G. (2005). Application of a novel widefield surface plasmon microscope in binding assays. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (Vol. 2005, pp. 140-142). Article 1548406 https://doi.org/10.1109/MMB.2005.1548406

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abstract = "The present work investigates the capabilities of a novel widefield surface plasmon microscope (WSPR) and its potential applications in biology. Here in particular, we concentrate on imaging interfacial interactions between specific antibodies and the proteins laminin and fibronectin, both patterned onto prefabricated Au / Cr / glass substrates. These experiments are used to demonstrate the high sensitivity, good contrast and high lateral resolution (close to a micron) of the new system. These results indicate that our microscope may form the basis for a new generation of fast assay systems.",

keywords = "Cell, Immunoassay, Surface plasmon, Widefield",

author = "S. Marlafeka and S. Liu and {Mahadi Abdul Jamil}, M. and Twigg, {P. C.} and Britland, {S. T.} and Denyer, {M. C.T.} and See, {C. W.} and Somekh, {Michael Geoffrey}",

year = "2005",

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doi = "10.1109/MMB.2005.1548406",

language = "English",

isbn = "0780387112",

volume = "2005",

pages = "140--142",

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Marlafeka, S, Liu, S, Mahadi Abdul Jamil, M, Twigg, PC, Britland, ST, Denyer, MCT, See, CW & Somekh, MG 2005, Application of a novel widefield surface plasmon microscope in binding assays. in 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. vol. 2005, 1548406, pp. 140-142, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, Oahu, HI, United States, 12/05/05. https://doi.org/10.1109/MMB.2005.1548406

Application of a novel widefield surface plasmon microscope in binding assays. / Marlafeka, S.; Liu, S.; Mahadi Abdul Jamil, M. et al.
2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. Vol. 2005 2005. p. 140-142 1548406.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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Application of a novel widefield surface plasmon microscope in binding assays

Abstract

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Keywords

ASJC Scopus subject areas

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