Abstract
The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.
| Original language | English |
|---|---|
| Pages (from-to) | 7-11 |
| Number of pages | 5 |
| Journal | Biosensors and Bioelectronics |
| Volume | 15 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1 Dec 2000 |
Keywords
- Fermentation
- Luminescence
- pH optrode
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
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