Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae

Chun Man Chan, Wai Hung Lo, Kwok Yin Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

16 Citations (Scopus)

Abstract

The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.
Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalBiosensors and Bioelectronics
Volume15
Issue number1-2
DOIs
Publication statusPublished - 1 Dec 2000

Keywords

  • Fermentation
  • Luminescence
  • pH optrode

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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