Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae

Chun Man Chan; Wai Hung Lo; Kwok Yin Wong

doi:10.1016/S0956-5663(00)00052-X

Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae

Chun Man Chan, Wai Hung Lo, Kwok Yin Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

17 Citations (Scopus)

Abstract

The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.

Original language	English
Pages (from-to)	7-11
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	15
Issue number	1-2
DOIs	https://doi.org/10.1016/S0956-5663(00)00052-X
Publication status	Published - 1 Dec 2000

Keywords

Fermentation
Luminescence
pH optrode

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/S0956-5663(00)00052-X

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title = "Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae",

abstract = "The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.",

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AU - Chan, Chun Man

AU - Lo, Wai Hung

AU - Wong, Kwok Yin

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Y1 - 2000/12/1

N2 - The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.

AB - The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+(bpy = 2,2′-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 μm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.

KW - Fermentation

KW - Luminescence

KW - pH optrode

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DO - 10.1016/S0956-5663(00)00052-X

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VL - 15

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EP - 11

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 1-2

ER -

Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae

Abstract

Keywords

ASJC Scopus subject areas

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