Antiproliferative effects of sclerotial polysaccharides from Polyporus rhinocerus cooke (Aphyllophoromycetideae) on different kinds of leukemic cells

Connie K M Lai, Ka Hing Wong, Peter Chi Keung Cheung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

49 Citations (Scopus)

Abstract

Mushroom polysaccharides isolated from fruiting bodies are known for their antitumor activities, whereas those from mushroom sclerotia are not. A hot water- (PR-HW) and a cold alkaline (PR-CA)-soluble polysaccharide isolated from a novel mushroom sclerotium, Polyporus rhinocerus Cooke, were characterized to be a polysaccharide-protein complex and a glucan, respectively. Their in vitro antitumor activities against different leukemic cell lines were investigated. PR-HW demonstrated significant growth inhibition of human acute promyelocytic leukemic cells (HL-60), chronic myelogenous leukemia cells (K562), and human acute monocytic leukemia cells (THP-1) in vitro, whereas PR-CA showed no such inhibition. Flow cytometric analysis has shown that the antiproliferative effect of PR-HW on HL-60 was mediated by cell cycle arrest at the G1 phase, which subsequently led to apoptosis. This suggests that structural differences in PR-HW and PR-CA greatly affects their antitumor activities.
Original languageEnglish
Pages (from-to)255-264
Number of pages10
JournalInternational Journal of Medicinal Mushrooms
Volume10
Issue number3
DOIs
Publication statusPublished - 28 Jul 2008
Externally publishedYes

Keywords

  • Antiproliferation
  • Cancer
  • Leukemic cells
  • Medicinal mushrooms
  • Polyporus rhinocerus
  • Polysaccharides
  • Sclerotium

ASJC Scopus subject areas

  • Molecular Medicine
  • Plant Science
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this