Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines

Ming Cheung Chow; Cheuk On Tang; I. T.N. Teo; C. H. Chui; F. Y. Lau; T. W.T. Leung; G. Cheng; R. S.M. Wong; I. L.K. Wong; K. M.S. Tsang; W. Q. Tan; Y. Z. Zhao; K. B. Lai; W. H. Lam; D. A. Guo; A. S.C. Chan

doi:10.1159/000069713

Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines

Ming Cheung Chow, Cheuk On Tang, I. T.N. Teo, C. H. Chui, F. Y. Lau, T. W.T. Leung, G. Cheng, R. S.M. Wong, I. L.K. Wong, K. M.S. Tsang, W. Q. Tan, Y. Z. Zhao, K. B. Lai, W. H. Lam, D. A. Guo, A. S.C. Chan

Research output: Journal article publication › Journal article › Academic research › peer-review

35 Citations (Scopus)

Abstract

Background: The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponinrich. However, its activity on solid tumour cell lines has never been demonstrated. Methods: The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection. Results: The mean MTT50(the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 μg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent. Conclusions: Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.

Original language	English
Pages (from-to)	303-308
Number of pages	6
Journal	Chemotherapy
Volume	48
Issue number	6
DOIs	https://doi.org/10.1159/000069713
Publication status	Published - 1 Dec 2002

Keywords

Apoptosis
Herbal drugs
Tumour

ASJC Scopus subject areas

Oncology
Pharmacology
Drug Discovery
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1159/000069713

Cite this

Chow, M. C., Tang, C. O., Teo, I. T. N., Chui, C. H., Lau, F. Y., Leung, T. W. T., Cheng, G., Wong, R. S. M., Wong, I. L. K., Tsang, K. M. S., Tan, W. Q., Zhao, Y. Z., Lai, K. B., Lam, W. H., Guo, D. A., & Chan, A. S. C. (2002). Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines. Chemotherapy, 48(6), 303-308. https://doi.org/10.1159/000069713

@article{1d4d665741bd4d0b9ac2786e36e3c492,

title = "Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines",

abstract = "Background: The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponinrich. However, its activity on solid tumour cell lines has never been demonstrated. Methods: The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection. Results: The mean MTT50(the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 μg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent. Conclusions: Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.",

keywords = "Apoptosis, Herbal drugs, Tumour",

author = "Chow, {Ming Cheung} and Tang, {Cheuk On} and Teo, {I. T.N.} and Chui, {C. H.} and Lau, {F. Y.} and Leung, {T. W.T.} and G. Cheng and Wong, {R. S.M.} and Wong, {I. L.K.} and Tsang, {K. M.S.} and Tan, {W. Q.} and Zhao, {Y. Z.} and Lai, {K. B.} and Lam, {W. H.} and Guo, {D. A.} and Chan, {A. S.C.}",

year = "2002",

month = dec,

day = "1",

doi = "10.1159/000069713",

language = "English",

volume = "48",

pages = "303--308",

journal = "Chemotherapy",

issn = "0009-3157",

publisher = "S. Karger AG",

number = "6",

}

TY - JOUR

T1 - Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines

AU - Chow, Ming Cheung

AU - Tang, Cheuk On

AU - Teo, I. T.N.

AU - Chui, C. H.

AU - Lau, F. Y.

AU - Leung, T. W.T.

AU - Cheng, G.

AU - Wong, R. S.M.

AU - Wong, I. L.K.

AU - Tsang, K. M.S.

AU - Tan, W. Q.

AU - Zhao, Y. Z.

AU - Lai, K. B.

AU - Lam, W. H.

AU - Guo, D. A.

AU - Chan, A. S.C.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Background: The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponinrich. However, its activity on solid tumour cell lines has never been demonstrated. Methods: The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection. Results: The mean MTT50(the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 μg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent. Conclusions: Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.

AB - Background: The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponinrich. However, its activity on solid tumour cell lines has never been demonstrated. Methods: The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection. Results: The mean MTT50(the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 μg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent. Conclusions: Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.

KW - Apoptosis

KW - Herbal drugs

KW - Tumour

UR - http://www.scopus.com/inward/record.url?scp=0036967073&partnerID=8YFLogxK

U2 - 10.1159/000069713

DO - 10.1159/000069713

M3 - Journal article

C2 - 12673105

SN - 0009-3157

VL - 48

SP - 303

EP - 308

JO - Chemotherapy

JF - Chemotherapy

IS - 6

ER -

Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

More information

Other files and links

Fingerprint

Cite this