Antiproliferative activity of the extract of Gleditsia sinensis fruit on human solid tumour cell lines

Ming Cheung Chow, Cheuk On Tang, I. T.N. Teo, C. H. Chui, F. Y. Lau, T. W.T. Leung, G. Cheng, R. S.M. Wong, I. L.K. Wong, K. M.S. Tsang, W. Q. Tan, Y. Z. Zhao, K. B. Lai, W. H. Lam, D. A. Guo, A. S.C. Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

31 Citations (Scopus)

Abstract

Background: The fruit extract of Gleditsia sinensis Lam. (GSE) is a traditional herbal medicine that is saponinrich. However, its activity on solid tumour cell lines has never been demonstrated. Methods: The activity of GSE was demonstrated in four cancer cell lines (breast cancer MCF-7, MDA-MB231, hepatoblastoma HepG2 and oesophageal squamous carcinoma cell line SLMT-1) using MTT assay, anchorage-independent clonogenicity assay, DNA laddering and in situ cell death detection. Results: The mean MTT50(the mean concentration of GSE to reduce MTT activity by 50%) ranged from 16 to 20 μg/ml of GSE. An anchorage-independent clonogenicity assay showed that all of the four solid tumour cell lines gradually lost their regeneration potential after treatment with GSE, DNA fragmentation and TUNEL analysis demonstrated that the action of GSE is both dose- and time course-dependent. Conclusions: Our results suggest that GSE has a cytotoxic activity and can induce apoptosis in human solid tumour cell lines.
Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalChemotherapy
Volume48
Issue number6
DOIs
Publication statusPublished - 1 Dec 2002

Keywords

  • Apoptosis
  • Herbal drugs
  • Tumour

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Drug Discovery
  • Pharmacology (medical)
  • Infectious Diseases

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