An optimal magnetite immobilized pseudomonasputida 5-x cellsystem for Cu2+removal from industrial waste effluent

L. Wang, H. Chua, P. K. Wong, Wai Hung Lo, P. H. Yu, Y. G. Zhao

Research output: Journal article publicationJournal articleAcademic researchpeer-review

13 Citations (Scopus)


An optimal magnetite immobilized Pseudomonas putida 5-x cell system was developed to remove Cu2+from industrial waste effluent. Cu2+adsorption capacity of P. putida 5-x cultured in sulphate-limiting medium (SLM) was minimum in early log growth phase, and reached maximum in late stationary growth phase or early death phase. Pretreated cells by 0.6 N HCl could greatly enhance the adsorption capacity of biomass up to 85.6 mg/g and had no significant effect for the loss of P. putida 5-x cells during the pretreatment. In a semi-continuous biosorption system, the removal efficiency of Cu2+from wastewater reached 96%, and recovery efficiency of Cu2+was 95%, and the concentration in the recovery solution was 1.4 g/L using 0.6 N HCl as eluant. The mechanism of Cu2+adsorption by this type of biomass was studied by using the technique of transmission electron microscopy (TEM). Degradation of a peptidoglycan layer on the cell surface was observed after acidic pretreatment, but no further degradation appeared after the adsorption-desorption cycle. TEM and X-ray analysis also showed that Cu2+was mainly accumulated on the cell surface, so it was effectively desorpted by acidic treatment in the desorption process. The process of adsorption obeyed the Freundlich isotherm.
Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalWater Science and Technology
Issue number12
Publication statusPublished - 30 Aug 2000


  • Copper
  • Growth phase
  • Magnetite-immobilized cells
  • Pseudomonas putida
  • Sorption and desorption
  • TEM analysis

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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