An optical biosensor for the rapid determination of glucose in human serum

Xu Dong Wang, Ting Yao Zhou, Xi Chen, Kwok Yin Wong, Xiao Ru Wang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

46 Citations (Scopus)


An optical glucose biosensor based on the immobilization of glucose oxidase in a sensing film was developed. The sensing film consisted of an organically modified silicate film embedded with tri(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) perchlorate and a polyvinyl alcohol sol-gel matrix with immobilized glucose oxidase. A kinetic curve simulation method was employed for glucose measurements, as a result of which the measurement time was less than 1 min for each sample analysis. The detection limit (S/N = 3) of the glucose optical sensor was 3.6 × 10-6mol L-1with linear ranges from 0.00 to 0.50 mmol L-1(Y = 358.25X-2.93, R2= 0.9954) and 0.50 to 3.00 mmol L-1(Y = 430.12X-46.43, R2= 0.9972). In addition, the appearance of the sensing film, and effects of the amounts of immobilized enzyme, pH, temperature, ionic strength, and co-existing substances (including heavy metal ions and organic compounds) were investigated. The glucose values estimated by this optical biosensor correlated well with those determined using the conventional method for human serum samples.
Original languageEnglish
Pages (from-to)866-873
Number of pages8
JournalSensors and Actuators, B: Chemical
Issue number2
Publication statusPublished - 22 Feb 2008


  • Glucose optical biosensor
  • Glucose oxidase
  • Kinetic measurement
  • Serum

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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