TY - JOUR
T1 - An ionic liquid-functionalized near-infrared fluorescent hydrogel dressing for promoting wound healing and real-time monitoring hypochlorous acid at the diabetic wound site
AU - Zong, Yuange
AU - Liu, Pan
AU - Zha, Ruyan
AU - Zong, Beige
AU - Wang, Yanying
AU - Fang, Huaifang
AU - Wong, Wing Leung
AU - Li, Chunya
N1 - Funding Information:
This project was supported by the National Natural Science Foundation of China (Nos. 22074160 , 21874157 , 22004133 ), Fundamental Research Fund for the Central University of South-Central Minzu University (No. CZZ22003 ), Health and Medical Research Fund , Hong Kong SAR (Project No. 19200231 and 22210412 ), Hong Kong Polytechnic University Startup Research Fund ( P0043754 ) and Chongqing Natural Science Foundation of China ( cstc2021jcyj-msxmX0670 ), and Open Project Funding of Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University (No. KLSAOFM2305 ). All experiments on animals were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals of South-Central Minzu University and were approved by the Institutional Ethics Committee of South-Central Minzu University (No. 2020-scuec-045 ). The operator has already passed the professional technical examination for laboratory animals (No. TY20201031 ). All experimental animals were in good status according to the National Laboratory Animal Law (China).
Funding Information:
This project was supported by the National Natural Science Foundation of China (Nos. 22074160, 21874157, 22004133), Fundamental Research Fund for the Central University of South-Central Minzu University (No. CZZ22003), Health and Medical Research Fund, Hong Kong SAR (Project No. 19200231 and 22210412), Hong Kong Polytechnic University Startup Research Fund (P0043754) and Chongqing Natural Science Foundation of China (cstc2021jcyj-msxmX0670), and Open Project Funding of Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University (No. KLSAOFM2305). All experiments on animals were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals of South-Central Minzu University and were approved by the Institutional Ethics Committee of South-Central Minzu University (No. 2020-scuec-045). The operator has already passed the professional technical examination for laboratory animals (No. TY20201031). All experimental animals were in good status according to the National Laboratory Animal Law (China).
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/11/1
Y1 - 2023/11/1
N2 - Diabetic wound is one of the most common complications of diabetes and is difficult to heal. Therefore, diabetic wound commonly turns into chronic ulcers. Some severe patients may face amputation because there is not much clinical treatment option available currently. Hypochlorous acid (HClO) is a ubiquitous reactive oxygen species (ROS) that plays a pivotal role in the inflammatory response at multiple stages and is considered as an important biomarker for monitoring diabetic wound healing. However, real-time monitoring HClO concentration at the injury site in diabetic wound therapy is a challenge and has not been fully explored. In this study, a novel near-infrared (NIR) fluorescent probe, SCy-7, was grafted onto a functionalized polymeric ionic liquid (PIL) and that was cross-linked with a modified hyaluronic acid (HA) to develop a near-infrared fluorescent smart hydrogel dressing (PIL-HA). This new PIL-HA hydrogel possessing excellent adhesion and mechanical properties, good biocompatibility, and highly effective antibacterial property can target the HClO formed at the wound site and followed generate fluorescence signal for real-time quantifying the concentration HClO. Furthermore, the function of PIL-HA smart dressing not only offers real-time monitoring and assessing HClO at diabetic wound sites via NIR fluorescent imaging, but also promoting the diabetic wound repair process. The in vivo therapeutic efficacy and toxicity of PIL-HA smart dressing were also evaluated with a diabetic mouse model.
AB - Diabetic wound is one of the most common complications of diabetes and is difficult to heal. Therefore, diabetic wound commonly turns into chronic ulcers. Some severe patients may face amputation because there is not much clinical treatment option available currently. Hypochlorous acid (HClO) is a ubiquitous reactive oxygen species (ROS) that plays a pivotal role in the inflammatory response at multiple stages and is considered as an important biomarker for monitoring diabetic wound healing. However, real-time monitoring HClO concentration at the injury site in diabetic wound therapy is a challenge and has not been fully explored. In this study, a novel near-infrared (NIR) fluorescent probe, SCy-7, was grafted onto a functionalized polymeric ionic liquid (PIL) and that was cross-linked with a modified hyaluronic acid (HA) to develop a near-infrared fluorescent smart hydrogel dressing (PIL-HA). This new PIL-HA hydrogel possessing excellent adhesion and mechanical properties, good biocompatibility, and highly effective antibacterial property can target the HClO formed at the wound site and followed generate fluorescence signal for real-time quantifying the concentration HClO. Furthermore, the function of PIL-HA smart dressing not only offers real-time monitoring and assessing HClO at diabetic wound sites via NIR fluorescent imaging, but also promoting the diabetic wound repair process. The in vivo therapeutic efficacy and toxicity of PIL-HA smart dressing were also evaluated with a diabetic mouse model.
KW - Diabetic wound therapy
KW - Functionalized ionic liquid
KW - Hyaluronic acid
KW - Hypochlorous acid monitoring
KW - Near-infrared fluorescent dressing
UR - http://www.scopus.com/inward/record.url?scp=85166911148&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2023.134405
DO - 10.1016/j.snb.2023.134405
M3 - Journal article
AN - SCOPUS:85166911148
SN - 0925-4005
VL - 394
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 134405
ER -