An antioxidative galactomannan-protein complex isolated from fermentation broth of a medicinal fungus Cs-HK1

Xia Chen, Zhong Yang Ding, Wen Qiang Wang, Ka Chai Siu, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

10 Citations (Scopus)


A protein-containing polysaccharide, EPS2BW, was fractionated from the exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis (Cs-HK1). EPS2BW was mainly composed of galactomannan with about 16% (w/w) protein and 50 kDa average molecular weight. The galactomannan part consisted of mannose and galactose at 1.7:1.0 molar ratio, and the protein segments were composed of sixteen amino acids with 12.5% proline and 16.6% threonine (mol%) being the most abundant. Based on analytical results from NMR, methylation analysis, partial acid hydrolysis and GC-MS, the galactomannan structure was elucidated as a (1 → 2)-α-d-mannopyranosyl (Manp) backbone with O-6-linked galactopyranosyl (Galp) branches. EPS2BW exhibited a high antioxidant capacity in both chemical and cell culture assays, with a Trolox equivalent radical scavenging activity of 44.7 μmol Trolox/mg, a Fe3+reducing power of 38.9 μmol Fe2+/mg, and significant cytoprotective effect against H2O2-induced PC12 cell death at 50-250 μg/mL.
Original languageEnglish
Pages (from-to)469-474
Number of pages6
JournalCarbohydrate Polymers
Publication statusPublished - 4 Nov 2014


  • Antioxidation
  • Cordyceps sinensis
  • Cytoprotection
  • Exopolysaccharides
  • Galactomannan
  • Polysaccharide-protein complex

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Medicine(all)

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