Amplitude and phase microscopy for sizing of spherical particles

Nicolas B.E. Sawyer, Stephen P. Morgan, Michael Geoffrey Somekh, Chung W. See, Eugene Astrakharchik-Farrimond, Boris Y. Shekunov

Research output: Journal article publicationJournal articleAcademic researchpeer-review

12 Citations (Scopus)


We describe a numerical vector diffraction model based on Mie theory that describes the imaging of spherical particles by bright-field, confocal, and interferometric microscopes. The model correctly scales the amplitude-scattered field relative to the incident field so that the forward-scattered and incident light can be interfered to correctly model imaging with copolarization transmission microscopes for the first time to our knowledge. The model is used to demonstrate that amplitude and phase imaging with an interferometric microscope allows subwavelength particle sizing. Furthermore, we show that the phase channel allows much smaller particles to be sized than amplitude-only measurements. The model is validated by experimental measurements.
Original languageEnglish
Pages (from-to)4488-4498
Number of pages11
JournalApplied Optics
Issue number22
Publication statusPublished - 1 Aug 2003
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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