Amplitude and phase microscopy for sizing of spherical particles

Nicolas B.E. Sawyer; Stephen P. Morgan; Michael Geoffrey Somekh; Chung W. See; Eugene Astrakharchik-Farrimond; Boris Y. Shekunov

doi:10.1364/AO.42.004488

Amplitude and phase microscopy for sizing of spherical particles

Nicolas B.E. Sawyer, Stephen P. Morgan, Michael Geoffrey Somekh, Chung W. See, Eugene Astrakharchik-Farrimond, Boris Y. Shekunov

Research output: Journal article publication › Journal article › Academic research › peer-review

12 Citations (Scopus)

Abstract

We describe a numerical vector diffraction model based on Mie theory that describes the imaging of spherical particles by bright-field, confocal, and interferometric microscopes. The model correctly scales the amplitude-scattered field relative to the incident field so that the forward-scattered and incident light can be interfered to correctly model imaging with copolarization transmission microscopes for the first time to our knowledge. The model is used to demonstrate that amplitude and phase imaging with an interferometric microscope allows subwavelength particle sizing. Furthermore, we show that the phase channel allows much smaller particles to be sized than amplitude-only measurements. The model is validated by experimental measurements.

Original language	English
Pages (from-to)	4488-4498
Number of pages	11
Journal	Applied Optics
Volume	42
Issue number	22
DOIs	https://doi.org/10.1364/AO.42.004488
Publication status	Published - 1 Aug 2003
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/AO.42.004488

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AU - Sawyer, Nicolas B.E.

AU - Morgan, Stephen P.

AU - Somekh, Michael Geoffrey

AU - See, Chung W.

AU - Astrakharchik-Farrimond, Eugene

AU - Shekunov, Boris Y.

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AB - We describe a numerical vector diffraction model based on Mie theory that describes the imaging of spherical particles by bright-field, confocal, and interferometric microscopes. The model correctly scales the amplitude-scattered field relative to the incident field so that the forward-scattered and incident light can be interfered to correctly model imaging with copolarization transmission microscopes for the first time to our knowledge. The model is used to demonstrate that amplitude and phase imaging with an interferometric microscope allows subwavelength particle sizing. Furthermore, we show that the phase channel allows much smaller particles to be sized than amplitude-only measurements. The model is validated by experimental measurements.

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ER -

Amplitude and phase microscopy for sizing of spherical particles

Abstract

ASJC Scopus subject areas

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