Amphiphilic core-shell nanoparticles with poly(ethylenimine) shells as potential gene delivery carriers

Junmin Zhu, Angie Tang, Lai Pang Law, Min Feng, Kin Man Ho, Daniel K.L. Lee, Frank W. Harris, Pei Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

124 Citations (Scopus)

Abstract

Spherical, well-defined core-shell nanoparticles that consist of poly(methyl methacrylate) (PMMA) cores and branched poly(ethylenimine) shells (PEI) were synthesized via a graft copolymerization of methyl methacrylate from branched PEI induced by a small amount of teri-butyl hydroperoxide. The PMMA-PEI core-shell nanoparticles were between 130 to 170 nm in diameter and displayed zeta-potentials near +40 mV at pH 7 in 1 mM aqueous NaCl. Plasmid DNA (pDNA) was mixed with nanoparticles and formed complexes of approximately 120 nm in diameter and was highly monodispersed. The complexes were characterized with respect to their particle size, zeta-potential, surface morphology, and DNA integrity. The complexing ability of the nanoparticles was strongly dependent on the molecular weight of the PEI and the thickness of the PEI shells. The stability of the complexes was influenced by the loading ratio of the pDNA and the nanoparticles. The condensed pDNA in the complexes was significantly protected from enzymatic degradation by DNase I. Cytotoxity studies using MTT colorimetric assays suggested that the PMMA-PEI (25 kDa) core-shell nanoparticles were three times less toxic than the branched PEI (25 kDa). Their transfection efficiencies were also significantly higher. Thus, the PEI-based core-shell nanoparticles show considerable potential as carriers for gene delivery.
Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalBioconjugate Chemistry
Volume16
Issue number1
DOIs
Publication statusPublished - 1 Jan 2005

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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