Aggregation-induced emission (AIE) dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation

Rui Hu, Chengbin Yang, Yucheng Wang, Guimiao Lin, Wei Qin, Qingling Ouyan, Wing Cheung Law, Quoc Toan Nguyen, Ho Sup Yoon, Xiaomei Wang, Ken Tye Yong, Ben Zhong Tang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

28 Citations (Scopus)

Abstract

We have developed aggregation-induced emission (AIE) dye loaded polymer nanoparticles with deep-red emission for siRNA delivery to pancreatic cancer cells. Two US Food and Drug Administration (FDA) approved surfactant polymers, Pluronics F127 and PEGylated phospholipid, were used to prepare the dye-loaded nanoparticle formulations and they can be used as nanovectors for gene silencing of mutant K-ras in pancreatic cancer cells. The successful transfection of siRNA by the developed nanovectors was confirmed by the fluorescent imaging and quantified through flow cytometry. Quantitative real time polymerase chain reaction (PCR) indicates that the expression of the mutant K-ras oncogene from the MiaPaCa-2 pancreatic cancer cells has been successfully suppressed. More importantly, our in vivo toxicity study has revealed that both the nanoparticle formulations are highly biocompatible in BALC/c mice. Overall, our results suggest that the AIE dye-loaded polymer nanoparticle formulations developed here are suitable for gene delivery and have high potential applications in translational medicine research.
Original languageEnglish
Pages (from-to)1563-1576
Number of pages14
JournalNano Research
Volume8
Issue number5
DOIs
Publication statusPublished - 18 May 2015

Keywords

  • aggregation-induced emission
  • gene silencing
  • pancreatic cancer
  • polymer nanoparticles

ASJC Scopus subject areas

  • Materials Science(all)
  • Electrical and Electronic Engineering

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