Advances in photoacoustic imaging reconstruction and quantitative analysis for biomedical applications

Photoacoustic imaging (PAI), a modality that combines the high contrast of optical imaging with the deep penetration of ultrasound, is rapidly transitioning from preclinical research to clinical practice. However, its widespread clinical adoption faces challenges such as the inherent trade-off between penetration depth and spatial resolution, along with the demand for faster imaging speeds. This review comprehensively examines the fundamental principles of PAI, focusing on three primary implementations: photoacoustic computed tomography, photoacoustic microscopy, and photoacoustic endoscopy. It critically analyzes their respective advantages and limitations to provide insights into practical applications. The discussion then extends to recent advancements in image reconstruction and artifact suppression, where both conventional and deep learning (DL)-based approaches have been highlighted for their role in enhancing image quality and streamlining workflows. Furthermore, this work explores progress in quantitative PAI, particularly its ability to precisely measure hemoglobin concentration, oxygen saturation, and other physiological biomarkers. Finally, this review outlines emerging trends and future directions, underscoring the transformative potential of DL in shaping the clinical evolution of PAI.