Adaptive responses of 25-hydroxyvitamin D31-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats

Wan Ping Lai; Tsui Shan Chau; Pik Yuen Cheung; Wen Fang Chen; Chun Lap Samuel Lo; Murray J. Favus; Man Sau Wong

doi:10.1016/S0925-4439(03)00123-6

Adaptive responses of 25-hydroxyvitamin D₃1-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats

Wan Ping Lai, Tsui Shan Chau, Pik Yuen Cheung, Wen Fang Chen, Chun Lap Samuel Lo, Murray J. Favus, Man Sau Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

6 Citations (Scopus)

Abstract

Regulation of vitamin D metabolism alters with age. The present study is undertaken to investigate if the loss of renal 1,25-dihydroxyvitamin D3(1,25(OH)2D3) production in response to dietary phosphate (P) restriction in adult rats is due to an alteration in the renal expression of 25-hydroxyvitamin D31-alpha hydroxylase (1-OHase). Young (4-6 weeks old) and adult (12-14 weeks old) male Sprague Dawley rats were fed either normal P (NPD) or low P diet (LPD) for 0-5 days. Basal expression of 1-OHase protein was higher in adult rats. Young rats, but not adult rats, significantly increased 1-OHase protein and mRNA expressions in response to LPD in a time-dependent manner. To determine if the stability of renal 1-OHase protein changes with LPD feeding, young and adult rats fed either NPD or LPD for 5 days were injected intravenously with cycloheximide (CHX), a protein synthesis inhibitor. CHX decreased 1-OHase protein expression in young rats fed NPD. However, CHX did not alter 1-OHase protein expression in young rats fed LPD nor in adult rats fed either diet. The results indicate that the stability of renal 1-OHase protein increased with age and that LPD increased its stability only in young rats.

Original language	English
Pages (from-to)	34-42
Number of pages	9
Journal	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volume	1639
Issue number	1
DOIs	https://doi.org/10.1016/S0925-4439(03)00123-6
Publication status	Published - 1 Sept 2003

Keywords

25-Hydroxyvitamin D 1-alpha hydroxylase protein 3
Aging
CYP27B mRNA expression
Low phosphate diet (LPD)
Renal proximal tubule

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology

More information

10.1016/S0925-4439(03)00123-6

Cite this

@article{61f119ddcd9449249f2646ab35daa9f8,

title = "Adaptive responses of 25-hydroxyvitamin D31-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats",

abstract = "Regulation of vitamin D metabolism alters with age. The present study is undertaken to investigate if the loss of renal 1,25-dihydroxyvitamin D3(1,25(OH)2D3) production in response to dietary phosphate (P) restriction in adult rats is due to an alteration in the renal expression of 25-hydroxyvitamin D31-alpha hydroxylase (1-OHase). Young (4-6 weeks old) and adult (12-14 weeks old) male Sprague Dawley rats were fed either normal P (NPD) or low P diet (LPD) for 0-5 days. Basal expression of 1-OHase protein was higher in adult rats. Young rats, but not adult rats, significantly increased 1-OHase protein and mRNA expressions in response to LPD in a time-dependent manner. To determine if the stability of renal 1-OHase protein changes with LPD feeding, young and adult rats fed either NPD or LPD for 5 days were injected intravenously with cycloheximide (CHX), a protein synthesis inhibitor. CHX decreased 1-OHase protein expression in young rats fed NPD. However, CHX did not alter 1-OHase protein expression in young rats fed LPD nor in adult rats fed either diet. The results indicate that the stability of renal 1-OHase protein increased with age and that LPD increased its stability only in young rats.",

keywords = "25-Hydroxyvitamin D 1-alpha hydroxylase protein 3, Aging, CYP27B mRNA expression, Low phosphate diet (LPD), Renal proximal tubule",

author = "Lai, {Wan Ping} and Chau, {Tsui Shan} and Cheung, {Pik Yuen} and Chen, {Wen Fang} and Lo, {Chun Lap Samuel} and Favus, {Murray J.} and Wong, {Man Sau}",

year = "2003",

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doi = "10.1016/S0925-4439(03)00123-6",

language = "English",

volume = "1639",

pages = "34--42",

journal = "Biochimica et Biophysica Acta - Molecular Basis of Disease",

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Adaptive responses of 25-hydroxyvitamin D₃1-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats. / Lai, Wan Ping; Chau, Tsui Shan; Cheung, Pik Yuen et al.
In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1639, No. 1, 01.09.2003, p. 34-42.

Research output: Journal article publication › Journal article › Academic research › peer-review

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T1 - Adaptive responses of 25-hydroxyvitamin D31-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats

AU - Lai, Wan Ping

AU - Chau, Tsui Shan

AU - Cheung, Pik Yuen

AU - Chen, Wen Fang

AU - Lo, Chun Lap Samuel

AU - Favus, Murray J.

AU - Wong, Man Sau

PY - 2003/9/1

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N2 - Regulation of vitamin D metabolism alters with age. The present study is undertaken to investigate if the loss of renal 1,25-dihydroxyvitamin D3(1,25(OH)2D3) production in response to dietary phosphate (P) restriction in adult rats is due to an alteration in the renal expression of 25-hydroxyvitamin D31-alpha hydroxylase (1-OHase). Young (4-6 weeks old) and adult (12-14 weeks old) male Sprague Dawley rats were fed either normal P (NPD) or low P diet (LPD) for 0-5 days. Basal expression of 1-OHase protein was higher in adult rats. Young rats, but not adult rats, significantly increased 1-OHase protein and mRNA expressions in response to LPD in a time-dependent manner. To determine if the stability of renal 1-OHase protein changes with LPD feeding, young and adult rats fed either NPD or LPD for 5 days were injected intravenously with cycloheximide (CHX), a protein synthesis inhibitor. CHX decreased 1-OHase protein expression in young rats fed NPD. However, CHX did not alter 1-OHase protein expression in young rats fed LPD nor in adult rats fed either diet. The results indicate that the stability of renal 1-OHase protein increased with age and that LPD increased its stability only in young rats.

AB - Regulation of vitamin D metabolism alters with age. The present study is undertaken to investigate if the loss of renal 1,25-dihydroxyvitamin D3(1,25(OH)2D3) production in response to dietary phosphate (P) restriction in adult rats is due to an alteration in the renal expression of 25-hydroxyvitamin D31-alpha hydroxylase (1-OHase). Young (4-6 weeks old) and adult (12-14 weeks old) male Sprague Dawley rats were fed either normal P (NPD) or low P diet (LPD) for 0-5 days. Basal expression of 1-OHase protein was higher in adult rats. Young rats, but not adult rats, significantly increased 1-OHase protein and mRNA expressions in response to LPD in a time-dependent manner. To determine if the stability of renal 1-OHase protein changes with LPD feeding, young and adult rats fed either NPD or LPD for 5 days were injected intravenously with cycloheximide (CHX), a protein synthesis inhibitor. CHX decreased 1-OHase protein expression in young rats fed NPD. However, CHX did not alter 1-OHase protein expression in young rats fed LPD nor in adult rats fed either diet. The results indicate that the stability of renal 1-OHase protein increased with age and that LPD increased its stability only in young rats.

KW - 25-Hydroxyvitamin D 1-alpha hydroxylase protein 3

KW - Aging

KW - CYP27B mRNA expression

KW - Low phosphate diet (LPD)

KW - Renal proximal tubule

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DO - 10.1016/S0925-4439(03)00123-6

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C2 - 12943966

SN - 0925-4439

VL - 1639

SP - 34

EP - 42

JO - Biochimica et Biophysica Acta - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta - Molecular Basis of Disease

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