TY - JOUR
T1 - Activation of multiple signaling pathways during the differentiation of mesenchymal stem cells cultured in a silicon nanowire microenvironment
AU - Liu, Dandan
AU - Yi, Changqing
AU - Fong, Chi Chun
AU - Jin, Qinghui
AU - Wang, Zuankai
AU - Yu, Wai Kin
AU - Sun, Dong
AU - Zhao, Jianlong
AU - Yang, Mengsu
N1 - Funding Information:
Funding: This work was supported by the General Research Fund of Hong Kong Research Grant Council (CityU-104411). National Basic Research Program of China (973. 2012CB933302), and the Key Laboratory Funding Scheme of Shenzhen Municipal Government, China.
PY - 2014/8
Y1 - 2014/8
N2 - Mesenchymal stem cells (MSC) offer an optimal source for bone tissue engineering due to their capability of undergoing multilineage differentiation, where the mechanical properties of the microenvironment of MSCs are vital for osteochondral formation. However, the mechanisms of how mechanical and microenvironmental cues control osteogenesis and chondrogenesis are yet to be elucidated. In this study, we investigated the effects of vertically aligned silicon nanowire (SiNW) array on the differentiation of MSCs and the associated molecular mechanisms involved in osteogenesis and chandrogenesis. The results showed that the microenvironment of SiNW array activated a number of mechanosensitive pathways (including Integrin, TGF-β/BMP, Akt, MAPK, Insulin, and Wnt pathways) in MSCs, which converged to stimulate the osteogenesis and chondrogenesis via the Ras-Raf-MEK-ERK cascade. From the Clinical Editor: This study reports on the mechanisms and microenvironmental influence of osteogenesis and chondrogenesis by mesenchymal stem cells interacting with vertically aligned silicon nanowire scaffolds.
AB - Mesenchymal stem cells (MSC) offer an optimal source for bone tissue engineering due to their capability of undergoing multilineage differentiation, where the mechanical properties of the microenvironment of MSCs are vital for osteochondral formation. However, the mechanisms of how mechanical and microenvironmental cues control osteogenesis and chondrogenesis are yet to be elucidated. In this study, we investigated the effects of vertically aligned silicon nanowire (SiNW) array on the differentiation of MSCs and the associated molecular mechanisms involved in osteogenesis and chandrogenesis. The results showed that the microenvironment of SiNW array activated a number of mechanosensitive pathways (including Integrin, TGF-β/BMP, Akt, MAPK, Insulin, and Wnt pathways) in MSCs, which converged to stimulate the osteogenesis and chondrogenesis via the Ras-Raf-MEK-ERK cascade. From the Clinical Editor: This study reports on the mechanisms and microenvironmental influence of osteogenesis and chondrogenesis by mesenchymal stem cells interacting with vertically aligned silicon nanowire scaffolds.
KW - Mechanical stimuli
KW - Mesenchymal stem cell
KW - Microenvironment
KW - Signaling pathways
KW - Silicon nanowire array
UR - http://www.scopus.com/inward/record.url?scp=84905262095&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2014.02.003
DO - 10.1016/j.nano.2014.02.003
M3 - Journal article
C2 - 24566272
AN - SCOPUS:84905262095
SN - 1549-9634
VL - 10
SP - 1153
EP - 1163
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 6
ER -