Abstract
Background: Mast cells are a potent source of mediators that regulate the inflammatory response in allergy and asthma. Mast cells can be activated through different receptors, for example, via cross-linkage of the high-affinity IgE receptor (FcεRI) and by adenosine acting on specific receptors. We have recently described mast cell survival of an IgE receptor activation by up-regulation of the anti-apoptotic gene A1. Objective: To compare mast cell survival and expression of A1 after activation through the FcεRI and by an adenosine agonist. Methods: Bone marrow-derived, cultured mouse mast cells (BMCMC) were activated either with IgE+antigen or with the adenosine receptor agonist 5′-N-ethylcarboxamido adenosine (NECA). Release of β-hexosaminidase, cell viability, phosphorylation of Akt and IkB-α, and expression of pro-survival and pro-apoptotic genes were measured after activation. Results: Activation of BMCMC with NECA caused the release of β-hexosaminidase, although to a lesser extent than after FcεRI activation (33% and 98%, respectively). Activation by both NECA and FcεRI stimulated phosphorylation of Akt (Ser473 and Thr308) and IkB-α (Ser32), both of which are implicated in the regulation of cell survival. However, only cells that were activated through FcεRI, but not by NECA, expressed A1 and exhibited an increased survival rate compared to the control. Conclusion: These results show that adenosine receptor activation of BMCMC does not induce the same survival programme in mast cells as does activation through FcεRI. These findings may be important for understanding the role that mast cells play in asthma provoked by different stimuli.
Original language | English |
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Pages (from-to) | 1135-1140 |
Number of pages | 6 |
Journal | Clinical and Experimental Allergy |
Volume | 33 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1 Aug 2003 |
Externally published | Yes |
Keywords
- A1
- Adenosine
- Akt
- Apoptosis
- Asthma
- De-granulation
- FcεRI
- IgE
- Mast cells
- Survival
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology