@article{643407b7f948405eb9c43e653e7f1494,
title = "Aconitine disrupts serotonin neurotransmission via 5-hydroxytryptamine receptor in zebrafish embryo",
abstract = "Medicinal plants of the genus Aconitum are one of the most commonly used herbs in traditional medicine in East Asia to treat conditions related to the heart, pain, or inflammation. However, these herbs are also dangerous as accidental poisoning due to misuse is a recurring issue. These plants contain a number of diester-diterpenoid alkaloid compounds and aconitine is the most abundant and active one. This study investigated neurotoxicity of aconitine to zebrafish embryos in early development in relation to serotonin regulation. Experimental results showed that aconitine exposure (1, 10, and 100 μM) increased frequency of coiling behavior in zebrafish embryos in a dose-dependent manner and this effect can be triggered by either exposure to 5-hydroxytryptamine 1A (5-HT1A) receptor agonist (±)-8-hydroxy-2-(dipropylamino)tetralin (8-OH-DPAT) or overexpression of serotonin receptor 5-htr1ab. At the same time, coiling behavior caused by aconitine exposure could be rescued by co-exposure to 5-HT1A receptor antagonist WAY-100635 Maleate (WAY100635) and knockdown of 5-htr1ab using morpholino. Exposure to aconitine also significantly increased serotonin receptor 5-htr1ab and 5-htr1bd gene expression at 24 h post fertilization (hpf), but decreased their expression and protein expression of the serotonin receptor at 96 hpf with the high dose. These results suggest that neurotoxicity caused by aconitine is mediated through the 5-HT receptor.",
keywords = "Aconitum, coiling behavior, neurotoxicity, serotonin, zebrafish embryos",
author = "Hao Chen and Feng Wang and Xuan Ni and Yi Rigui and Yuxia Bai and Liang Xu and Jingfeng Yang and Xuefu Zhang and Jiang Deng and Jiawei Li and Xiaoyu Yin and Wuliji Ao and Kwok, {Kevin W.H.} and Wu Dong",
note = "Funding Information: Open Project Program of Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology China, Grant/Award Number: MDK2019051; Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China, Grant/Award Numbers: MDK2017014, MDK2018027, MDK2019074; Inner Mongolia University for Nationalities, China, Grant/Award Number: MDXK008; Inner Mongolia Grassland Talent Project, China; Natural Science Foundation of Inner Mongolia Autonomous Region of China, Grant/Award Number: 2020MS08103; National Natural Science Foundation of China, Grant/Award Numbers: 21267015, 21567019, 81360508 Funding information Funding Information: This work was funded by the National Natural Science Foundation of China (21267015, 21567019, and 81360508), the Natural Science Foundation of Inner Mongolia Autonomous Region of China (2020MS08103), Inner Mongolia Grassland Talent Project, China (2020) to Dr. Wu Dong, Inner Mongolia University for Nationalities, China (MDXK008), Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China (MDK2017014, MDK2018027, and MDK2019074), and Open Project Program of Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology China (MDK2019051). Publisher Copyright: {\textcopyright} 2020 John Wiley & Sons, Ltd.",
year = "2021",
month = mar,
doi = "10.1002/jat.4059",
language = "English",
volume = "41",
pages = "483--492",
journal = "Journal of Applied Toxicology",
issn = "0260-437X",
publisher = "John Wiley and Sons Ltd",
number = "3",
}