@inbook{b62e9590857845679fee82e96fbf479e,
title = "A zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation",
abstract = "The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.",
keywords = "Hematopoiesis, Mutation, Myeloid malignancy, Myelopoiesis, Transient expression, Zebrafish",
author = "Ma, {Chun Hang} and Xiangguo Shi and He, {Bai Liang} and Yuhan Guo and Leung, {Anskar Y.H.}",
year = "2017",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-7142-8_13",
language = "English",
series = "Methods in Molecular Biology",
pages = "193--218",
booktitle = "Methods in Molecular Biology",
}