A zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation

Chun Hang Ma, Xiangguo Shi, Bai Liang He, Yuhan Guo, Anskar Y.H. Leung

Research output: Chapter in book / Conference proceedingChapter in an edited book (as author)Academic researchpeer-review

1 Citation (Scopus)

Abstract

The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages193-218
Number of pages26
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1633
ISSN (Print)1064-3745

Keywords

  • Hematopoiesis
  • Mutation
  • Myeloid malignancy
  • Myelopoiesis
  • Transient expression
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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