A vortex pump-based optically-transparent microfluidic platform for biotech and medical applications

Kin Fong Lei, Wing Cheung Law, Yick Keung Suen, Wen J. Li, Yeung Yam, Ho Pui Ho, Siu Kai Kong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

12 Citations (Scopus)

Abstract

This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor that demonstrates the detection of specific binding of biomolecules and that qualitatively monitors cell adhesion on the sensor surface. Micropumps, microchannels, and an SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied for its potential applications in bio-molecules detection and drugs discovery. Two experiments, (1) monitoring the reaction between the BSA-BSA antibody, and (2) monitoring the activities of living cells in the presence or absence of trypsin in a RPMI-1640 medium, were conducted to show the biomedical application capability. Because SPR based bio-detection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass in microfluidic systems, if bio-compatibility and low-cost are desired. Hence, our work has shown the feasibility of commercializing an SPR based bio-medical/ chemical analysis system in the near future.
Original languageEnglish
Pages (from-to)129-141
Number of pages13
JournalProceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine
Volume221
Issue number2
DOIs
Publication statusPublished - 1 Jan 2007
Externally publishedYes

Keywords

  • Bio-medical microfluids
  • Microfluidic systems
  • SPR biosensor

ASJC Scopus subject areas

  • Mechanical Engineering

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