TY - JOUR
T1 - A time-resolved near-infrared phosphorescent iridium(iii) complex for fast and highly specific peroxynitrite detection and bioimaging applications
AU - Li, Yuanyan
AU - Wu, Yongquan
AU - Chen, Luyan
AU - Zeng, Hong
AU - Chen, Xiaoyong
AU - Lun, Weican
AU - Fan, Xiaolin
AU - Wong, Wai Yeung
PY - 2019/10/29
Y1 - 2019/10/29
N2 - Peroxynitrite (ONOO-), one of the reactive oxygen/nitrogen species (ROS/RNS) found in vivo, plays crucial roles in many physiological and pathological processes. The ability to selectively and sensitively determine ONOO-in vivo is important for the understanding of its biological roles. Thus, by utilizing the excellent chemical stability and photostability, high luminescence efficiency, and long luminescence lifetime of iridium complexes, we developed a novel near-infrared (NIR) phosphorescent iridium(iii) complex (FNO2) probe to detect ONOO- within seconds. The probe FNO2 showed better selectivity towards ONOO- over other interfering biomolecules, including O2- and ClO-. Moreover, it possessed a long luminescence lifetime, which enabled successful elimination of the interference from background fluorescence in vitro (simulated by Rhodamine B) in time-resolved emission spectra. Finally, in addition to its low cytotoxicity, the probe FNO2 showed emission wavelength in the NIR region and was able to specifically sense ONOO- induced in living cells and inflamed mouse models.
AB - Peroxynitrite (ONOO-), one of the reactive oxygen/nitrogen species (ROS/RNS) found in vivo, plays crucial roles in many physiological and pathological processes. The ability to selectively and sensitively determine ONOO-in vivo is important for the understanding of its biological roles. Thus, by utilizing the excellent chemical stability and photostability, high luminescence efficiency, and long luminescence lifetime of iridium complexes, we developed a novel near-infrared (NIR) phosphorescent iridium(iii) complex (FNO2) probe to detect ONOO- within seconds. The probe FNO2 showed better selectivity towards ONOO- over other interfering biomolecules, including O2- and ClO-. Moreover, it possessed a long luminescence lifetime, which enabled successful elimination of the interference from background fluorescence in vitro (simulated by Rhodamine B) in time-resolved emission spectra. Finally, in addition to its low cytotoxicity, the probe FNO2 showed emission wavelength in the NIR region and was able to specifically sense ONOO- induced in living cells and inflamed mouse models.
UR - http://www.scopus.com/inward/record.url?scp=85076005818&partnerID=8YFLogxK
U2 - 10.1039/c9tb01673b
DO - 10.1039/c9tb01673b
M3 - Journal article
C2 - 31746928
AN - SCOPUS:85076005818
SN - 2050-750X
VL - 7
SP - 7612
EP - 7618
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 47
ER -