A surface-charge study on cellular-uptake behavior of F3- peptide-conjugated iron oxide nanoparticles

Yu Zhang, Mo Yang, Ji Ho Park, Jennifer Singelyn, Huiqing Ma, Michael J. Sailor, Erkki Ruoslahti, Mihrimah Ozkan, Cengiz Ozkan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

86 Citations (Scopus)


Surface-charge measurements of mammalian cells in terms of Zeta potential are demonstrated as a useful biological characteristic in identifying cellular interactions with specific nanomaterials. A theoretical model of the changes in Zeta potential of cells after incubation with nanoparticles is established to predict the possible patterns of Zeta-potential change to reveal the binding and internalization effects. The experimental results show a distinct pattern of Zeta potential change that allows the discrimination of human normal breast epithelial cells (MCF-10A) from human cancer breast epithelial cells (MCF-7) when the cells are incubated with dextran coated iron oxide nanoparticles that contain tumor-homing F3 peptides, where the tumor-homing F3 peptide specifically bound to nucleolin receptors that are overexpressed in cancer breast cells.
Original languageEnglish
Pages (from-to)1990-1996
Number of pages7
Issue number17
Publication statusPublished - 4 Sep 2009


  • Cellular uptake
  • F peptide 3
  • Iron oxide nanoparticles
  • Zeta potential

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

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