Abstract
Surface-charge measurements of mammalian cells in terms of Zeta potential are demonstrated as a useful biological characteristic in identifying cellular interactions with specific nanomaterials. A theoretical model of the changes in Zeta potential of cells after incubation with nanoparticles is established to predict the possible patterns of Zeta-potential change to reveal the binding and internalization effects. The experimental results show a distinct pattern of Zeta potential change that allows the discrimination of human normal breast epithelial cells (MCF-10A) from human cancer breast epithelial cells (MCF-7) when the cells are incubated with dextran coated iron oxide nanoparticles that contain tumor-homing F3 peptides, where the tumor-homing F3 peptide specifically bound to nucleolin receptors that are overexpressed in cancer breast cells.
Original language | English |
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Pages (from-to) | 1990-1996 |
Number of pages | 7 |
Journal | Small |
Volume | 5 |
Issue number | 17 |
DOIs | |
Publication status | Published - 4 Sept 2009 |
Keywords
- Cellular uptake
- F peptide 3
- Iron oxide nanoparticles
- Zeta potential
ASJC Scopus subject areas
- Biotechnology
- Biomaterials
- Engineering (miscellaneous)