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A study of axonal protein trafficking in neuronal networks via the microfluidic platform

  • Y. Fu
  • , A. Vandongen
  • , T. Bourouina
  • , W. M. Tsang
  • , M. Je
  • , A. Q. Liu

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

This paper reports a microfluidic platform for culturing neuronal networks and the study of axonal protein trafficking. The device consists of cultural microchambers connected by microchannel array. Molecular biological methods were used to express fluorescent proteins in neurons. Preliminary results show that the neurons can be polarized with their soma and axons being compartmentalized into different fluidically isolated microenvironments. When chemical stimulation was applied to axonal chamber, anterograde migration of expressed fluorescent proteins could be observed. The platform will be useful in neuroscience research for studying the response of individual neurons to extracellular stimulation.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1072-1074
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Nov 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Axonal protein
  • Microfluidic
  • Neuron
  • Protein trafficking

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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