TY - JOUR
T1 - A standardized extract of Danggui Buxue Tang decoction selectively exerts estrogenic activities distinctly from tamoxifen
AU - Zhou, Liping
AU - Wong, Ka Ying
AU - Cao, Sisi
AU - Poon, Christina Chui Wa
AU - Yu, Wenxuan
AU - Dong, Xiaoli
AU - Tsim, Karl Wah Keung
AU - Wong, Man Sau
N1 - Funding Information:
Health and Medical Research Fund, Grant/Award Number: 11122111 Funding information Radix Astragali Radix Angelicae Sinensis
Funding Information:
This work was supported by the Health and Medical Research Fund (HMRF) grant (Ref. No. 11122111) of Hong Kong. We thank the University Research Facility in Life Sciences at the Hong Kong Polytechnic University for the technical support.
Funding Information:
This work was supported by the Health and Medical Research Fund (HMRF) grant (Ref. No. 11122111) of Hong Kong. We thank the University Research Facility in Life Sciences at the Hong Kong Polytechnic University for the technical support.
Publisher Copyright:
© 2020 John Wiley & Sons Ltd
PY - 2021/3
Y1 - 2021/3
N2 - More and more menopausal women use Danggui Buxue Tang (DBT) for relieving their symptoms. Concerns for its safety have been raised as it contains phytoestrogen and acts via estrogen receptors (ERs). Our study aimed to determine whether DBT could selectively exert estrogenic activities and interact with tamoxifen in bone, brain, uterus, and breast by using ovariectomized (OVX) rats and ER-positive cells. In OVX rats, DBT induced a 31.4% increase in bone mineral density and restored the mRNA expression of dopamine biomarker in striatum, 3.32-fold for tyrosine hydrolase (p <.001) and 0.21-fold for dopamine transporter (p <.001), which was similar to tamoxifen; tamoxifen, but not DBT, increased uterus weight and Complement component 3 expression by more than twofold (p <.001); unlike tamoxifen, DBT induced mild proliferation in mammary gland. Two-way ANOVA indicated the interactions between them in OVX rats (p <.05) but DBT did not alter the responses to tamoxifen. DBT stimulated proliferation or differentiation and estrogen response element in MCF-7, MG-63, Ishikawa, and SHSY5Y cells and altered the effects of tamoxifen. In summary, DBT exerted estrogenic effects in tissue-selective manner, which was different from tamoxifen. DBT interacted with tamoxifen but did not significantly alter its effects in OVX rats.
AB - More and more menopausal women use Danggui Buxue Tang (DBT) for relieving their symptoms. Concerns for its safety have been raised as it contains phytoestrogen and acts via estrogen receptors (ERs). Our study aimed to determine whether DBT could selectively exert estrogenic activities and interact with tamoxifen in bone, brain, uterus, and breast by using ovariectomized (OVX) rats and ER-positive cells. In OVX rats, DBT induced a 31.4% increase in bone mineral density and restored the mRNA expression of dopamine biomarker in striatum, 3.32-fold for tyrosine hydrolase (p <.001) and 0.21-fold for dopamine transporter (p <.001), which was similar to tamoxifen; tamoxifen, but not DBT, increased uterus weight and Complement component 3 expression by more than twofold (p <.001); unlike tamoxifen, DBT induced mild proliferation in mammary gland. Two-way ANOVA indicated the interactions between them in OVX rats (p <.05) but DBT did not alter the responses to tamoxifen. DBT stimulated proliferation or differentiation and estrogen response element in MCF-7, MG-63, Ishikawa, and SHSY5Y cells and altered the effects of tamoxifen. In summary, DBT exerted estrogenic effects in tissue-selective manner, which was different from tamoxifen. DBT interacted with tamoxifen but did not significantly alter its effects in OVX rats.
KW - Danggui Buxue Tang decoction
KW - estrogen receptors
KW - estrogenic activities
KW - phytoestrogen
KW - selective estrogen receptor modulators (SERMs)
KW - tissue-selectivity
UR - http://www.scopus.com/inward/record.url?scp=85092563586&partnerID=8YFLogxK
U2 - 10.1002/ptr.6909
DO - 10.1002/ptr.6909
M3 - Journal article
C2 - 33063371
AN - SCOPUS:85092563586
SN - 0951-418X
VL - 35
SP - 1456
EP - 1467
JO - Phytotherapy Research
JF - Phytotherapy Research
IS - 3
ER -